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SHR Neuro Cancer Cardio Lipid Metab Microb
Köhler, J; Breitbach, K; Renner, C; Heitsch, AK; Bast, A; van Rooijen, N; Vogelgesang, S; Steinmetz, I.
NADPH-oxidase but not inducible nitric oxide synthase contributes to resistance in a murine Staphylococcus aureus Newman pneumonia model.
Microbes Infect. 2011; 13(11):914-922
Doi: 10.1016/j.micinf.2011.05.004
Web of Science
PubMed
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FullText_MUG
- Leading authors Med Uni Graz
- Steinmetz Ivo
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- Abstract:
- Staphylococcus aureus is a pathogen that often causes severe nosocomial infections including pneumonia. The present study was designed to examine innate phagocyte mediated immune mechanisms using a previously described murine S. aureus Newman pneumonia model. We found that BALB/c mice represent a more susceptible mouse strain compared to C57BL/6 mice after intranasal S. aureus Newman challenge. Depletion experiments revealed that neutrophils are a crucial determinant for resistance whereas depletion of alveolar macrophages protected mice to some degree from acute pulmonary S. aureus challenge. C57BL/6 mice lacking the subunit gp91phox of the NADPH-oxidase (gp91phox⁻/⁻ mice) proved to be highly susceptible against the pathogen. In contrast, C57BL/6 inducible nitric oxidase synthase deficient (iNOS⁻/⁻) mice did not differ in their clinical outcome after infection. Neither bone marrow macrophages from iNOS-/- nor from gp91phox⁻/⁻ mice were impaired in controlling intracellular persistence of S. aureus. Our data suggest that neutrophil and NADPH-oxidase mediated mechanisms are essential components in protecting the host against pulmonary S. aureus Newman challenge. On contrary, macrophages as well as NO mediated mechanisms do not seem to play a critical role for resistance in this model. Copyright © 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Disease Models, Animal -
- Female -
- Macrophages - immunology
- Macrophages - microbiology
- Mice -
- Mice, Inbred BALB C -
- Mice, Inbred C57BL -
- Mice, Knockout -
- NADPH Oxidases - immunology
- NADPH Oxidases - metabolism
- Nitric Oxide Synthase Type II - immunology
- Nitric Oxide Synthase Type II - metabolism
- Pneumonia, Staphylococcal - immunology
- Rodent Diseases - immunology
- Staphylococcus aureus - immunology
- Find related publications in this database (Keywords)
- Staphylococcus aureus
- Macrophage
- Neutrophil
- Nitric oxide
- NADPH-oxidase