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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Egemnazarov, B; Schmidt, A; Crnkovic, S; Sydykov, A; Nagy, BM; Kovacs, G; Weissmann, N; Olschewski, H; Olschewski, A; Kwapiszewska, G; Marsh, LM.
Pressure Overload Creates Right Ventricular Diastolic Dysfunction in a Mouse Model: Assessment by Echocardiography.
J Am Soc Echocardiogr. 2015; 28(7):828-843
Doi: 10.1016/j.echo.2015.02.014
Web of Science
PubMed
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FullText_MUG
- Leading authors Med Uni Graz
- Egemnazarov Bakytbek
- Co-authors Med Uni Graz
- Crnkovic Slaven
- Kovacs Gabor
- Kwapiszewska-Marsh Grazyna
- Marsh Leigh
- Nagy Miklos Bence
- Olschewski Andrea
- Olschewski Horst
- Schmidt Albrecht
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- Abstract:
- Noninvasive diagnostic tools for right ventricular (RV) dysfunction measurements are increasingly being used, although their association with the pathologic mechanisms of dysfunction is poorly understood. Although investigations have focused mainly on RV systolic function, RV diastolic function remains mostly neglected. The aim of this study was to test which echocardiographic parameters best reflect RV diastolic function in mice. Pulmonary artery banding (PAB) was used to induce RV pressure overload in mice. Transthoracic echocardiography and invasive hemodynamic measurements were performed after 3 weeks in PAB and sham-operated mice. Subsequently, the hearts were investigated by histology and analyzed for gene expression. PAB-induced pressure overload (RV systolic pressure PAB 52.6 ± 11.8 mm Hg vs sham 27.0 ± 2.7 mm Hg) resulted in RV hypertrophy and remodeling, as reflected by increased Fulton index (PAB 0.37 ± 0.05 vs sham 0.25 ± 0.02, P = .001). Masson's trichrome staining revealed increased interstitial fibrosis (PAB 12.25 ± 3.12% vs sham 3.97 ± 1.58%, P = .002). This was associated with significant systolic RV dysfunction as demonstrated by reduced contractility index and diastolic dysfunction as demonstrated by end-diastolic pressure (PAB 2.66 ± 0.83 mm Hg vs sham 1.49 ± 0.50 mm Hg, P < .001) and τ (PAB 40.0 ± 16.1 msec vs sham 13.0 ± 3.5 msec, P < .001). Messenger ribonucleic acid expression of β-myosin heavy chain, atrial and brain natriuretic peptides, collagen family members was elevated, and the sarco/endoplasmic reticulum Ca(2+)-ATPase was decreased. Echocardiography revealed significant increases in RV free wall thickness and isovolumic relaxation time and a decrease in left ventricular eccentricity index, E', and tricuspid annular plane systolic excursion. Isovolumic relaxation time and E' were significantly correlated with end-diastolic pressure (rs = 0.511 and -0.451) and τ (rs = 0.739 and -0.445, respectively). Moreover, E' was negatively correlated with the degree of RV fibrosis (rs = -0.717). Within 3 weeks, PAB causes pressure overload-induced RV hypertrophy and remodeling with compensated systolic and diastolic dysfunction in mice. RV free wall thickness, tricuspid annular plane systolic excursion, E', E/E' ratio, and isovolumic relaxation time appear to be the most reliable echocardiographic parameters for the assessment of RV dysfunction. Copyright © 2015 American Society of Echocardiography. Published by Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Diastole -
- Disease Models, Animal -
- Echocardiography - methods
- Heart Ventricles - diagnostic imaging
- Heart Ventricles - physiopathology
- Male -
- Mice -
- Mice, Inbred C57BL -
- Ventricular Dysfunction, Right - diagnostic imaging
- Ventricular Dysfunction, Right - physiopathology
- Ventricular Function, Right - physiology
- Ventricular Pressure - physiology
- Find related publications in this database (Keywords)
- Right ventricle
- Fibrosis
- Echocardiography
- Dysfunction
- Diastolic dysfunction
- Pressure overload