Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Sreckovic, I; Birner-Gruenberger, R; Besenboeck, C; Miljkovic, M; Stojakovic, T; Scharnagl, H; Marsche, G; Lang, U; Kotur-Stevuljevic, J; Jelic-Ivanovic, Z; Desoye, G; Wadsack, C.
Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity.
Biochim Biophys Acta. 2014; 1841(11):1619-1627
Doi: 10.1016/j.bbalip.2014.07.021
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Birner-Grünberger Ruth
- Sreckovic Ivana
- Wadsack Christian
- Co-authors Med Uni Graz
- Desoye Gernot
- Lang Uwe
- Marsche Gunther
- Scharnagl Hubert
- Schliefsteiner Carolin
- Stojakovic Tatjana
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Gestational diabetes mellitus (GDM) is related to neonatal macrosomia and an increased risk of vascular events. We hypothesized that GDM exerts qualitative effects on neonatal high-density lipoprotein (HDL). HDL was isolated from control (n=11) and GDM maternal/neonatal donors (n=9) and subjected to shotgun proteomics. Differences in HDL mobility were assessed by FPLC and native gel-electrophoresis. Paraoxonase (PON1) activity, cholesterol ester-transfer protein (CETP) mass and activity, phospholipid, triglyceride and cholesterol concentrations were quantified with commercial kits. Total anti-oxidative capacity and cholesterol efflux capability of HDLs were measured. Four proteins involved in lipid metabolism, inflammation and innate immunity were differentially expressed between controls and GDM neonates. ApoM (decreased, p<0.05) and SAA1 (increased, p<0.05) showed the same differences on both, maternal and neonatal GDM HDL. Lower PON1 protein expression was corroborated by lower activity (p<0.05) which in turn was associated with attenuated anti-oxidant capacity of GDM HDL. Protein changes were accompanied by increased levels of triglycerides and decreased levels of cholesterol esters, respectively. The observed differences in GDM HDL lipid moiety may be related to CETP mass and activity alterations. The rate of cholesterol efflux from term trophoblasts to maternal and from placental endothelial cells to neonatal GDM HDL was impaired (p<0.05). In conclusion, GDM causes changes in HDL composition and is intimately associated with impaired cholesterol efflux capability as well as diminished anti-oxidative particle properties. Remodeling of neonatal GDM HDL in utero supports the hypothesis that maternal conditions in pregnancy impact neonatal lipoprotein metabolism. Copyright © 2014. Published by Elsevier B.V.
- Find related publications in this database (Keywords)
- Neonatal HDL
- GDM
- Anti-oxidative property