Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Kap, M; Arron, GI; Loibner, M; Hausleitner, A; Siaulyte, G; Zatloukal, K; Murk, JL; Riegman, P.
Inactivation of Influenza A virus, Adenovirus, and Cytomegalovirus with PAXgene tissue fixative and formalin.
Biopreserv Biobank. 2013; 11(4):229-234
Doi: 10.1089/bio.2013.0010
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Hausleitner Anja
- Loibner Martina
- Siaulyte Gintare
- Zatloukal Kurt
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- Abstract:
- Formalin fixation is known to inactivate most viruses in a vaccine production context, but nothing is published about virus activity in tissues treated with alternative, non-crosslinking fixatives. We used a model assay based on cell culture to test formalin and PAXgene Tissue fixative for their virus-inactivating abilities. MDCK, A549, and MRC-5 cells were infected with Influenza A virus, Adenovirus, and Cytomegalovirus, respectively. When 75% of the cells showed a cytopathic effect (CPE), the cells were harvested and incubated for 15 min, or 1, 3, 6, or 24 hours, with PBS (positive control), 4% formalin, or PAXgene Tissue Fix. The cells were disrupted and the released virus was used to infect fresh MDCK, A549, and MRC-5 cells cultured on cover slips in 24-well plates. The viral cultures were monitored for CPE and by immunocytochemistry (ICC) to record viral replication and infectivity. Inactivation of Adenovirus by formalin occurred after 3 h, while Influenza A virus as well as Cytomegalovirus were inactivated by formalin after 15 min. All three virus strains were inactivated by PAXgene Tissue fixative after 15 min. We conclude that PAXgene Tissue fixative is at least as effective as formalin in inactivating infectivity of Influenza A virus, Adenovirus, and Cytomegalovirus.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenoviridae - drug effects
- Animals -
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- Cytomegalovirus - drug effects
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- Fixatives -
- Formaldehyde - pharmacology
- Humans -
- Influenza A virus - drug effects
- Madin Darby Canine Kidney Cells -
- Tissue Fixation - methods
- Virus Inactivation - drug effects
- Virus Replication - drug effects