Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Heinzle, A; Papen-Botterhuis, NE; Schiffer, D; Schneider, KP; Binder, B; Schintler, M; Haaksman, IK; Lenting, HB; Gübitz, GM; Sigl, E.
Novel protease-based diagnostic devices for detection of wound infection.
Wound Repair Regen. 2013; 21(3):482-489
Doi: 10.1111/wrr.12040
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Binder Barbara
- Schintler Michael
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- Abstract:
- A gelatinase-based device for fast detection of wound infection was developed. Collective gelatinolytic activity in infected wounds was 23 times higher (p ≤ 0.001) than in noninfected wounds and blisters according to the clinical and microbiological description of the wounds. Enzyme activities of critical wounds showed 12-fold elevated enzyme activities compared with noninfected wounds and blisters. Upon incubation of gelatin-based devices with infected wound fluids, an incubation time of 30 minutes led to a clearly visible dye release. A 32-fold color increase was measured after 60 minutes. Both matrix metalloproteinases and elastases contributed to collective gelatinolytic enzyme activity as shown by zymography and inhibition experiments. The metalloproteinase inhibitor 1,10-phenanthroline (targeting matrix metalloproteinases) and the serine protease inhibitor phenylmethlysulfonyl fluoride (targeting human neutrophil elastase) inhibited gelatinolytic activity in infected wound fluid samples by 11-37% and 60-95%, respectively. Staphylococcus aureus and Pseudomonas aeruginosa, both known for gelatinase production, were isolated in infected wound samples.
- Find related publications in this database (using NLM MeSH Indexing)
- Bacteria - enzymology
- Equipment Design -
- Humans -
- Microbiological Techniques - instrumentation
- Peptide Hydrolases - biosynthesis
- Reproducibility of Results -
- Wound Infection - diagnosis