Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Hasmann, A; Wehrschuetz-Sigl, E; Marold, A; Wiesbauer, H; Schoeftner, R; Gewessler, U; Kandelbauer, A; Schiffer, D; Schneider, KP; Binder, B; Schintler, M; Guebitz, GM.
Analysis of myeloperoxidase activity in wound fluids as a marker of infection.
Ann Clin Biochem. 2013; 50(Pt 3):245-254
Doi: 10.1258/acb.2011.010249
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- Co-authors Med Uni Graz
- Binder Barbara
- Geweßler Ulrike
- Marold Annemarie
- Schintler Michael
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- Abstract:
- Neutrophilic polymorphonuclear leukocytes play a crucial role in the host defence against bacterial and fungal infections. They participate in the inflammatory response through the liberation of peptides and enzymes like myeloperoxidase (MPO). Therefore, MPO has a potential as a marker enzyme for the diagnosis of wound infection. Substrate specificities and reaction pathways of MPO were investigated for new MPO substrates: crystal violet, leuco crystal violet, fast blue RR (4-benzoylamino-2,5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt) and various systematically substituted model substrates based on 2,7-dihydroxy-1-(4-hydroxyphenylazo)naphtalene-3,6-disulphonic acid. In addition, fast blue RR was covalently bound to siloxanes allowing immobilization of the substrate, while cellobiosedehydrogenase was integrated for generation of hydrogen peroxide required by MPO. Elevated concentrations of MPO were found in infected wounds compared with non-infected wounds (92.2 ± 45.0 versus 1.9 ± 1.8 U/mL). Various soluble and immobilized substrates were oxidized by MPO in wound samples and the influence of substrate structure and reaction pathways were elucidated for selected compounds. Incubation of different MPO substrates with infected wound fluid samples resulted in a clear colour change in the case of elevated MPO concentrations, thus allowing early diagnosis of wound infection.
- Find related publications in this database (using NLM MeSH Indexing)
- Biomarkers - analysis
- Body Fluids - enzymology
- Body Fluids - metabolism
- Enzyme Assays -
- Humans -
- Hydrogen Peroxide -
- Neutrophils - enzymology
- Neutrophils - metabolism
- Oxidation-Reduction -
- Peroxidase - analysis
- Wound Infection - diagnosis
- Wound Infection - enzymology