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Selected Publication:
Tiran, A; Tiran, B; Hojas, S; Kostner, GM; Wilders-Truschnig, MM.
Immunoquantification of lipoprotein(a): comparison of nephelometry with electroimmunodiffusion.
J Clin Lab Anal. 1993; 7(5):256-262
Doi: 10.1002/jcla.1860070504
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Tiran Andreas
- Co-authors Med Uni Graz
- Kostner Gerhard
- Tiran Beate
- Truschnig-Wilders Martini
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- Abstract:
- A new fully automated nephelometric immunoassay for lipoprotein(a) quantification in human serum was evaluated using the Behring Nephelometer Analyzer. The assay exhibited a good linearity in the concentration range of 110-1,770 mg/l; at higher concentrations, samples were automatically diluted by a factor of 4. The method is simple, robust, and shows an excellent stability of the calibration curve over several weeks. Intra-assay and day-to-day coefficients of variation were 2% and 4.5%, respectively. The method correlated well with electroimmunodiffusion (r = 0.977; n = 123; P = 0.0001). Unspecific turbidity as expressed by an elevated blank value occurred in 3% of all freshly measured samples (n = 392). Storage of the samples for 1 week at 4 degrees C had no significant influence on the results. Frozen sera, on the other hand, cannot be assayed by this method. We believe that this assay is well suited for use in clinical routine work.
- Find related publications in this database (using NLM MeSH Indexing)
- Calibration -
- Humans -
- Immune Sera - immunology
- Immunoassay - methods
- Immunodiffusion - methods
- Lipoprotein(a) - analysis
- Nephelometry and Turbidimetry - analysis
- Sensitivity and Specificity - analysis
- Find related publications in this database (Keywords)
- Lipoprotein(A) Quantification
- Behring Nephelometer Analyzer
- Electroimmunodiffusion Analysis