Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Lang, I; Hartmann, M; Blaschitz, A; Dohr, G; Skofitsch, G; Desoye, G.
Immunohistochemical evidence for the heterogeneity of maternal and fetal vascular endothelial cells in human full-term placenta.
CELL TISSUE RES 1993 274: 211-218.
Doi: 10.1007/BF00318740
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- Führende Autor*innen der Med Uni Graz
- Lang-Olip Ingrid
- Co-Autor*innen der Med Uni Graz
- Blaschitz Astrid
- Desoye Gernot
- Dohr Gottfried
- Hartmann Michaele
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- Abstract:
- The heterogeneity of endothelial cell surface antigen expression was studied in 5 human full-term placentae by means of indirect immunohistochemistry using 9 monoclonal antibodies and by staining with fluorescent-conjugated Ulex europaeus lectin, both of which are widely used endothelial cell markers. (1) A highly specific, homogeneous staining of fetal and maternal placental vessels of all sizes and anatomical regions was observed by the monoclonal antibodies PAL-E, QBEND10 and 1F10. These antibodies were even more specific than Ulex europaeus lectin, factor VIII antibody and von Willebrand factor antibody, which cross-reacted with some non-endothelial cells and structures. The reactivity of PAL-E, QBEND10 and 1F10 with residual surface cells of the basal plate strongly suggests an endothelial origin of these cells. (2) In contrast to other organs, PAL-E, QBEND10 and HM15/3 strongly stained endothelial cells of the macrovascular system in the human placenta. This might indicate an organ-associated heterogeneity of fetal endothelial cells. (3) Monoclonal antibodies against receptors for transferrin and IgG (Fc gamma RII) labeled the endothelial cells of fetal placental vessels with increasing intensity distal to the insertion of the umbilical cord. The vessels of the umbilical cord itself were unreactive. This might suggest a heterogeneity of macro- and microvascular endothelial cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies, Monoclonal - immunology
- Biological Markers - immunology
- Endothelium, Vascular - cytology
- Factor VIII - analysis
- Female - analysis
- Fetus - cytology
- Humans - cytology
- Isoantigens - analysis
- Lectins - analysis
- Organ Specificity - analysis
- Placenta - blood supply
- Plant Lectins - blood supply
- Pregnancy - blood supply
- Pregnancy Proteins - analysis
- Receptors, IgG - analysis
- Receptors, Transferrin - analysis
- Staining and Labeling - analysis
- Umbilical Cord - cytology
- Vimentin - analysis
- Find related publications in this database (Keywords)
- Umbilical Cord
- Placenta
- Basal Plate
- Endothelial Cells
- Immunohistochemistry
- Lectins
- Human