Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Keck, M; Zeyda, M; Burjak, S; Kamolz, LP; Selig, H; Stulnig, TM; Frey, M.
Coenzyme q10 does not enhance preadipocyte viability in an in vitro lipotransfer model.
Aesthetic Plast Surg. 2012; 36(2):453-457
Doi: 10.1007/s00266-011-9823-8
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Kamolz Lars-Peter
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- Abstract:
- Autologous fat is an attractive soft-tissue filler in plastic and reconstructive surgery. The success of the procedure relies strongly on the technique of transferring viable preadipocytes. Among other factors, preadipocyte viability is impaired by local anesthetics. Application of coenzyme Q10 is being performed by aesthetic plastic surgeons to enhance the success of lipotransfer. The aim of this study was to evaluate the effect of Q10 on preadipocyte viability with special regard to impairment after lidocaine treatment. Preadipocytes were pretreated with coenzyme Q10 or vehicle control followed by incubation with lidocaine for 30 min. Viability and apoptosis were assessed by FACS analysis and Western blot. Coenzyme Q10 did not improve viability nor have any effect on investigated apoptosis parameters. Preadipocyte viability was reduced after lidocaine treatment. Surface binding of annexin V, cleavage of caspase-3, and abundance of subdiploid cells were not detectable though, suggesting that necrosis rather than apoptosis is the cause for reduced preadipocyte viability. Our results indicate that Q10 does not improve preadipocyte viability. Preadipocyte cell death induced by lidocaine is not caused by apoptosis but by necrosis, which cannot be prevented by coenzyme Q10. These findings should be taken into account when searching for solutions to improve preadipocyte viability in the context of soft tissue engineering and autologous fat transfer.
- Find related publications in this database (using NLM MeSH Indexing)
- Adipocytes - pathology
- Adipose Tissue - transplantation
- Anesthetics, Local - pharmacology
- Apoptosis -
- Cell Survival - drug effects
- Humans -
- Lidocaine - pharmacology
- Necrosis -
- Transplantation, Autologous -
- Ubiquinone - analogs & derivatives
- Find related publications in this database (Keywords)
- Autologous fat transplantation
- Apoptosis
- Necrosis
- Local anesthetic
- Coenzyme Q10