Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Amann, R; Maggi, CA; Giuliani, S; Donnerer, J; Lembeck, F.
Effects of carbonyl cyanide p-trichloromethoxyphenylhydrazone (CCCP) and of ruthenium red (RR) on capsaicin-evoked neuropeptide release from peripheral terminals of primary afferent neurones.
Naunyn Schmiedebergs Arch Pharmacol. 1990; 341(6):534-537
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- Co-Autor*innen der Med Uni Graz
- Donnerer Josef
- Lembeck Fred
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- Abstract:
- In the superfused isolated rat urinary bladder, capsaicin as well as electrical field stimulation evoked the release of calcitonin gene-related peptide-like immunoreactivity (CGRP-IR). Carbonyl cyanide p-trichloromethoxyphenylhydrazone (CCCP, threshold 2 microM) reduced both, the capsaicin- and the electrical field stimulation-evoked release of CGRP-IR while a low concentration of Ruthenium Red (RR, 0.6 microM and 2 microM) selectively attenuated the capsaicin-evoked release of CGRP-IR but did not influence the effect of electrical field stimulation. 20 microM RR nearly abolished the capsaicin-evoked release, but also attenuated the effect of electrical field stimulation. In the isolated guinea-pig bronchus, electrical field stimulation and capsaicin induced non-cholinergic contractions which are known to be caused by tachykinin release from afferent nerve terminals. CCCP (0.6 microM) only reduced the response to field stimulation; a ten-fold higher concentration of CCCP attenuated field stimulation as well as capsaicin-induced contractions. This is in contrast to the reported selective inhibition of capsaicin-induced contractions by RR. The present data demonstrate that CCCP generally inhibits evoked neuropeptide release, regardless of the kind of stimulation used while low concentrations of RR preferentially inhibit capsaicin-evoked neuropeptide release.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Bronchi - innervation
- Calcitonin Gene-Related Peptide - metabolism
- Capsaicin - antagonists and inhibitors
- Carbonyl Cyanide m-Chlorophenyl Hydrazone - pharmacology
- Electric Stimulation - pharmacology
- Guinea Pigs - pharmacology
- Male - pharmacology
- Nerve Endings - drug effects
- Neurokinin A - pharmacology
- Neurons, Afferent - drug effects
- Neuropeptides - metabolism
- Nitriles - pharmacology
- Rats - pharmacology
- Ruthenium - pharmacology
- Ruthenium Red - pharmacology
- Urinary Bladder - drug effects