Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Lembeck, F; Griesbacher, T; Eckhardt, M.
Demonstration of extrapulmonary activity of angiotensin converting enzyme in intact tissue preparations.
Br J Pharmacol. 1990; 100(1):49-54
Doi: 10.1111/j.1476-5381.1990.tb12050.x
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- Führende Autor*innen der Med Uni Graz
- Lembeck Fred
- Co-Autor*innen der Med Uni Graz
- Griesbacher Thomas
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- Abstract:
- 1. The activity of angiotensin converting enzyme (ACE) has been studied on functional parameters of intact isolated preparations of extrapulmonary tissues. The conversion of angiotensin I (A I) to angiotensin II (A II) and the cleavage of bradykinin (BK) were used as indicators of ACE activity. Captopril was employed as a specific inhibitor of ACE. 2. Captopril augmented the BK-induced contractions of the rat isolated uterus, the BK- and substance P-induced contractions of the guinea-pig ileum, and the BK-induced venoconstriction in the isolated perfused ear of the rabbit. Degradation of BK by ACE was calculated to be 52% in the rat uterus and 75% in the rabbit perfused ear. 3. Captopril inhibited the A I-induced contractions of the rat isolated colon, the A I-induced vasoconstriction in the isolated perfused ear of the rabbit and the rise in blood pressure induced by i.a. injections of A I in pithed rats. Conversion of A I to A II was calculated to be 13% in the rat colon and 26% in the rabbit perfused ear. 4. From estimations of the A II activity (bioassay on the rat colon) in the effluent of the perfused ear of the rabbit after injections of A I into the arterial inflow cannula it was calculated that approximately one tenth of A I was converted to A II during a single passage through the ear (less than 15 s).(ABSTRACT TRUNCATED AT 250 WORDS)
- Find related publications in this database (using NLM MeSH Indexing)
- Angiotensin II - metabolism
- Angiotensin-Converting Enzyme Inhibitors - pharmacology
- Animals - pharmacology
- Blood Pressure - drug effects
- Bradykinin - physiology
- Captopril - pharmacology
- Endothelium, Vascular - drug effects
- Female - drug effects
- Guinea Pigs - drug effects
- Ileum - drug effects
- Lung - metabolism
- Male - metabolism
- Muscle, Smooth - enzymology
- Muscle, Smooth, Vascular - enzymology
- Norepinephrine - pharmacology
- Peptidyl-Dipeptidase A - metabolism
- Rabbits - metabolism
- Rats - metabolism
- Rats, Inbred Strains - metabolism
- Uterine Contraction - drug effects
- Vasoconstriction - drug effects