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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Wolinski, H; Kolb, D; Hermann, S; Koning, RI; Kohlwein, SD.
A role for seipin in lipid droplet dynamics and inheritance in yeast.
J Cell Sci. 2011; 124(Pt 22): 3894-3904.
Doi: 10.1242/jcs.091454
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- Co-authors Med Uni Graz
- Kolb Dagmar
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- Abstract:
- Malfunctions of processes involved in cellular lipid storage and mobilization induce the pathogenesis of prevalent human diseases such as obesity, type 2 diabetes and atherosclerosis. Lipid droplets are the main lipid storage depots for neutral lipids in eukaryotic cells, and as such fulfil an essential function to balance cellular lipid metabolism and energy homeostasis. Despite significant progress in identifying key metabolic enzymes involved in lipid storage and their regulation in various model organisms, some fundamental questions as to the biogenesis, subcellular distribution and inheritance of lipid droplets are as yet unsolved. In this study, we applied a set of imaging techniques such as high-resolution four-dimensional (4D) live-cell imaging, quantitative microscopy, transmission electron microscopy and electron tomography to gain insight into the spatio-temporal organization of lipid droplets during cellular growth in the yeast Saccharomyces cerevisiae. This analysis revealed a high level of organization of the subcellular positioning of lipid droplets in individual cells, their directed migration towards the cellular periphery and a coordinated transfer of a subpopulation of lipid droplets into daughter cells during cell division. Lipid droplets appear to remain associated with ER membranes during cellular growth independently of their size and subcellular localization. Deletion of FLD1, the functional orthologue of the human BSCL2 gene encoding seipin, leads to impaired dynamics of yeast lipid droplets and defective lipolysis, which might be due to aberrant ER structures in these mutants. Our data suggest a role for yeast seipin as a scaffolding protein that is required for the dynamics of a specific subdomain of the ER, and provide a new aspect for the interpretation of abnormal lipid droplets phenotypes in yeast mutants lacking seipin.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Tracking -
- Endoplasmic Reticulum - metabolism
- GTP-Binding Protein gamma Subunits - genetics
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- Lipolysis -
- Saccharomyces cerevisiae - genetics
- Find related publications in this database (Keywords)
- Neutral lipid
- Lipolysis
- Lipid droplet
- Endoplasmic reticulum
- Fluorescence microscopy
- Electron microscopy
- Tomography
- Saccharomyces cerevisiae