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SHR Neuro Cancer Cardio Lipid Metab Microb
Daniele, T; Hackmann, Y; Ritter, AT; Wenham, M; Booth, S; Bossi, G; Schintler, M; Auer-Grumbach, M; Griffiths, GM.
A role for rab7 in the movement of secretory granules in cytotoxic T lymphocytes.
Traffic. 2011; 12(7): 902-911.
Doi: 10.1111/j.1600-0854.2011.01194.x
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- Co-authors Med Uni Graz
- Auer-Grumbach Michaela
- Schintler Michael
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- Abstract:
- Cytotoxic T lymphocytes (CTL) are potent killers of virally infected and tumorigenic cells. Upon recognition of target cells, CTL undergo polarized secretion of secretory lysosomes at the immunological synapse (IS) that forms between CTL and target. However, the molecular machinery involved in the polarization of secretory lysosomes is still largely uncharacterized. In this paper, we investigated the role of Rab7 in the polarization of secretory lysosomes. We show that silencing of Rab7 by RNA interference reduces the ability of CTL to kill targets. GTP-bound Rab7 and Rab interacting lysosomal protein, RILP, interact and both localize to secretory lysosomes in CTL. Over-expression of RILP recruits dynein to the membranes of secretory lysosomes and triggers their movement toward the centrosome. Together, these results suggest that Rab7 may play a role in secretory lysosome movement toward the centrosome by interacting with RILP to recruit the minus-end motor, dynein.
- Find related publications in this database (using NLM MeSH Indexing)
- Adaptor Proteins, Signal Transducing - genetics
- Animals -
- Carrier Proteins - metabolism
- Cytoskeletal Proteins - metabolism
- Dyneins - metabolism
- Guanosine Triphosphate - metabolism
- HeLa Cells -
- Humans -
- Lysosomes - metabolism
- Mice -
- Mice, Inbred C57BL -
- Mutation -
- RNA Interference -
- Recombinant Fusion Proteins - genetics
- Secretory Vesicles - metabolism
- T-Lymphocytes, Cytotoxic - metabolism
- rab GTP-Binding Proteins - genetics
- Find related publications in this database (Keywords)
- Bicaudal
- CTL
- CMT2B
- dynein
- lymphocytes
- lysosomes
- ORP1L
- polarization
- Rab7
- RILP
- secretion