Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Almer, G; Wernig, K; Saba-Lepek, M; Haj-Yahya, S; Rattenberger, J; Wagner, J; Gradauer, K; Frascione, D; Pabst, G; Leitinger, G; Mangge, H; Zimmer, A; Prassl, R.
Adiponectin-coated nanoparticles for enhanced imaging of atherosclerotic plaques.
Int J Nanomedicine. 2011; 6(1):1279-1290
Doi: 10.2147/IJN.S18739
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- Leading authors Med Uni Graz
- Almer Gunter
- Prassl Ruth
- Co-authors Med Uni Graz
- Gradauer Kerstin
- Haj-Yahya Samih
- Leitinger Gerd
- Mangge Harald
- Saba-Lepek Matthias
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- Abstract:
- Background: Atherosclerosis is a leading cause of mortality in the Western world, and plaque diagnosis is still a challenge in cardiovascular medicine. The main focus of this study was to make atherosclerotic plaques visible using targeted nanoparticles for improved imaging. Today various biomarkers are known to be involved in the pathophysiologic scenario of atherosclerotic plaques. One promising new candidate is the globular domain of the adipocytokine adiponectin (gAd), which was used as a targeting sequence in this study. Methods: gAd was coupled to two different types of nanoparticles, namely protamine-oligonucleotide nanoparticles, known as proticles, and sterically stabilized liposomes. Both gAd-targeted nanoparticles were investigated for their potency to characterize critical scenarios within early and advanced atherosclerotic plaque lesions using an atherosclerotic mouse model. Aortic tissue from wild type and apolipoprotein E-deficient mice, both fed a high-fat diet, were stained with either fluorescent-labeled gAd or gAd-coupled nanoparticles. Ex vivo imaging was performed using confocal laser scanning microscopy. Results: gAd-targeted sterically stabilized liposomes generated a strong signal by accumulating at the surface of atherosclerotic plaques, while gAd-targeted proticles became internalized and showed more spotted plaque staining. Conclusion: Our results offer a promising perspective for enhanced in vivo imaging using gAd-targeted nanoparticles. By means of nanoparticles, a higher payload of signal emitting molecules could be transported to atherosclerotic plaques. Additionally, the opportunity is opened up to visualize different regions in the plaque scenario, depending on the nature of the nanoparticle used.
- Find related publications in this database (using NLM MeSH Indexing)
- Adiponectin - chemistry Adiponectin - metabolism
- Animals -
- Biological Markers - analysis
- Contrast Media - chemistry Contrast Media - metabolism
- Drug Delivery Systems -
- Histocytochemistry -
- Liposomes - chemistry
- Mice -
- Mice, Inbred C57BL -
- Microscopy, Confocal -
- Microscopy, Electron, Transmission -
- Microscopy, Fluorescence -
- Molecular Imaging - methods
- Nanoparticles - chemistry Nanoparticles - ultrastructure
- Plaque, Atherosclerotic - diagnosis Plaque, Atherosclerotic - metabolism Plaque, Atherosclerotic - pathology
- Find related publications in this database (Keywords)
- adiponectin
- nanoparticles
- proticles
- liposomes
- molecular imaging
- atherosclerosis