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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Schlagenhauf, A; Schweintzger, S; Birner-Grünberger, R; Leschnik, B; Muntean, W.
Comparative evaluation of PAR1, GPIb-IX-V, and integrin αIIbβ3 levels in cord and adult platelets.
Hamostaseologie. 2010; 30 Suppl 1(1):S164-S167
Doi: 10.1055/s-0037-1619105
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Muntean Eugen
- Schlagenhauf Axel
- Co-authors Med Uni Graz
- Birner-Grünberger Ruth
- Leschnik Bettina
- Schweintzger Sabrina
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- Abstract:
- Background: Newborn platelets show hyposensitivity in vitro to many important agonists. However, sensitivity of platelets to these agonists is crucial for a functional clot formation. Nevertheless newborns have an excellent hemostasis. Hence, we examined levels of PAR1 thrombin receptor, GPIb-IX-V (CD42b), and Integrin αIIbβ3 in newborn and adult platelets using Western blot analysis. Materials, methods: Platelets of adult and cord blood were isolated, washed, and lysed. Protein samples were separated by SDS-PAGE and blotted on nitrocellulose membranes. Receptors were visualized using immunodetection and evaluated densitometrically. Statistical analysis was performed using SPSS 16.0. Results: We found significantly lower levels of PAR1-receptors and higher levels of CD42b in newborn platelets as compared to adult platelets. Levels of Integrin αIIbβ3 in newborn platelets were comparable to adult platelets. Conclusion: A lower content of PAR1-receptors explains very well the hyposensitivity of cord platelets to thrombin. Higher levels of CD42b may additionally support the effect of larger more adhesive multimeric vWF in newborn plasma.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Blood Platelets - metabolism
- Fetal Blood - metabolism
- Humans -
- Infant, Newborn -
- Phospholipids - blood
- Platelet Aggregation -
- Platelet Glycoprotein GPIIb-IIIa Complex - metabolism
- Platelet Glycoprotein GPIb-IX Complex - metabolism
- Receptors, Thrombin - metabolism
- Find related publications in this database (Keywords)
- Newborn haemostasis
- platelet receptors
- Western blot
- thrombin receptor