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Cervoni, F; Oglesby, TJ; Fénichel, P; Dohr, G; Rossi, B; Atkinson, JP; Hsi, BL.
Expression of decay-accelerating factor (CD55) of the complement system on human spermatozoa.
J Immunol. 1993; 151(2):939-948
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Co-authors Med Uni Graz: Dohr Gottfried
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Abstract:: Human spermatozoa were analyzed for their expression of decay-accelerating factor (DAF, CD55), a glycolipid-anchored regulatory protein of the C casade. Morphologic data showed that DAF was localized at the acrosomal region of the sperm head. Analysis with anti-DAF antibody-immunoprecipitated proteins of acrosome-reached spermatozoa revealed a 44- to 54-kDa protein. Carbohydrate analysis of sperm DAF indicated that it contains nonsialated N- and O-linked sugars. The absence of mature oligosaccharides on this protein appears to account for the difference in molecular mass between sperm DAF and the 70-kDa DAF expressed on other human tissues. Sperm DAF reinserted into sheep E and inhibited C-mediated lysis. This effect was reversed by mAb, which block DAF function. These results indicate that sperm DAF also possesses a glycolipid anchor. The expression of DAF on acrosome-reacted spermatozoa suggests that it may act concomitantly with other C regulators such as membrane cofactor protein to modulate the activation of C in the immunocompetent female genital tract and protect acrosome-reacted spermatozoa from C-mediated attack.
Find related publications in this database (using NLM MeSH Indexing): Acrosome - physiology; Animals - physiology; Antigens, CD - analysis; Antigens, CD46 - analysis; Antigens, CD55 - analysis; Carbohydrates - analysis; Complement Inactivator Proteins - analysis; Humans - analysis; Male - analysis; Membrane Glycoproteins - analysis; Rabbits - analysis; Sperm Head - immunology; Spermatozoa - immunology