Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Hammer, A; Hutter, H; Blaschitz, A; Mahnert, W; Hartmann, M; Uchanska-Ziegler, B; Ziegler, A; Dohr, G.
Amnion epithelial cells, in contrast to trophoblast cells, express all classical HLA class I molecules together with HLA-G.
Am J Reprod Immunol. 1997; 37(2):161-171
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- Führende Autor*innen der Med Uni Graz
- Hammer Astrid
- Co-Autor*innen der Med Uni Graz
- Blaschitz Astrid
- Dohr Gottfried
- Hartmann Michaele
- Hutter Heinz
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- Abstract:
- PROBLEM: The expression of the non-classical HLA-G gene has been shown at the protein level on trophoblast-derived embryonic tissue, like the extravillous cytotrophoblast. However, the presence of HLA-G on embryoblast-derived cells is currently controversial. The amnion epithelium is an embryoblast-derived cell layer covering the amnion cavity and is the main source for the amnion fluid. METHOD: The expression of HLA class I molecules was investigated by immunohistochemical, biochemical, and molecular biological methods in amnion membranes and amnion fluid. RESULTS: Immunohistochemically, HLA-C and occasionally also-B molecules as well as HLA-A and/or -G molecules have been identified on amnion epithelial cells. These results were extended by Western blotting with purified amnion epithelial cells where HLA-B and/or -C, HLA-A and HLA-G antigens have been detected. As expected HLA-G mRNA was detected in amino epithelial cells. Furthermore, classical HLA molecules as well as HLA-G were found in amnion fluid. CONCLUSION: These results show that the amnion epithelium frequently expresses classical HLA class I molecules as well as HLA-G. The expression of HLA-G antigens on amnion epithelial cells and their presence in the amnion fluid, which is continually ingested by the fetus, may be particularly relevant for the induction of peripheral tolerance.
- Find related publications in this database (using NLM MeSH Indexing)
- Amnion - chemistry
- Amniotic Fluid - chemistry
- Blotting, Northern - chemistry
- Blotting, Western - chemistry
- Cells, Cultured - chemistry
- Epithelial Cells - chemistry
- Epithelium - chemistry
- HLA Antigens - biosynthesis
- Histocompatibility Antigens Class I - biosynthesis
- Humans - biosynthesis
- Immunohistochemistry - biosynthesis
- Trophoblasts - cytology