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Valenta, R; Ball, T; Vrtala, S; Duchêne, M; Kraft, D; Scheiner, O.
cDNA cloning and expression of timothy grass (Phleum pratense) pollen profilin in Escherichia coli: comparison with birch pollen profilin.
Biochem Biophys Res Commun. 1994; 199(1):106-118
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Authors Med Uni Graz:
Ball Tanja

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Profilin, an actin-binding protein, was previously described as a ubiquitous allergen which is responsible for cross-reactivities in about 20% of pollen and food allergic patients. A complete cDNA clone coding for timothy grass (Phelum pratense) pollen profilin was isolated using allergic patients IgE. The deduced amino acid sequence of timothy grass profilin shares a sequence identity of 79% with birch profilin and other plant profilins and a lower average sequence identity of 35% with other eukaryotic profilins. The high degree of homology among different plant profilins at the DNA and protein level explains the extensive cross-reactivities observed in profilin allergic patients. Recombinant timothy grass pollen profilin was expressed in Escherichia coli as a beta-galactosidase fusion protein and shown to bind IgE from profilin allergic patients similar to recombinant birch profilin. Slight differences regarding the IgE-binding capacity of birch and timothy grass profilin indicate that not all IgE-epitopes of the two profilins are conserved. It is speculated that profilin allergic patients were initially sensitized against a certain profilin and then cross-react with the homologous proteins.
Find related publications in this database (using NLM MeSH Indexing)
Allergens - genetics
Base Sequence -
Cloning, Molecular -
Contractile Proteins -
DNA, Complementary - genetics
Humans -
Immunoglobulin E - metabolism
Microfilament Proteins - genetics Microfilament Proteins - immunology
Molecular Sequence Data -
Poaceae -
Pollen -
Profilins -
Sequence Alignment -
Sequence Homology, Amino Acid -
Sequence Homology, Nucleic Acid -
Solubility -
Trees -

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