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Ball, T; Vrtala, S; Sperr, WR; Valent, P; Susani, M; Kraft, D; Valenta, R.
Isolation of an immunodominant IgE hapten from an epitope expression cDNA library. Dissection of the allergic effector reaction.
J Biol Chem. 1994; 269(45):28323-28328 [OPEN ACCESS]
Web of Science PubMed


Authors Med Uni Graz:
Ball Tanja

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An epitope expression cDNA library was constructed from the randomly fragmented cDNA coding for Phl p I, the major grass pollen allergen. Using IgE from allergic patients, epitope clones were isolated and immunodominant fragments were selected. Among three epitope clones coding for a similar region of Phl p I, one clone expressed a 15-amino-acid epitope which was target for IgE antibodies from approximately 30% of grass pollen allergic patients. According to the prevalence of grass pollen allergy, 22% of all allergic patients are expected to display IgE reactivity with this epitope. Although the purified recombinant epitope specifically bound IgE, it did not release histamine from basophiles of most grass pollen allergic patients and thus represents an IgE hapten. Immunodominant IgE haptens may be useful as therapeutic agents to saturate mast cell-bound IgE prior to allergen exposure and may represent candidates for a safe immunotherapy of allergic diseases by reducing anaphylactic side effects.
Find related publications in this database (using NLM MeSH Indexing)
Allergens -
Amino Acid Sequence -
Antigen-Antibody Complex -
Base Sequence -
Basophils - drug effects Basophils - immunology
DNA, Complementary -
Epitopes - biosynthesis Epitopes - immunology Epitopes - isolation and purification
Escherichia coli -
Gene Library -
Haptens - biosynthesis Haptens - immunology Haptens - isolation and purification
Histamine Release - drug effects
Humans -
Immunoglobulin E - immunology
Molecular Sequence Data -
Oligodeoxyribonucleotides -
Peptide Fragments - immunology
Plant Proteins - biosynthesis Plant Proteins - immunology Plant Proteins - isolation and purification
Poaceae - immunology
Pollen -
Recombinant Proteins - biosynthesis Recombinant Proteins - immunology Recombinant Proteins - isolation and purification
Sequence Homology, Amino Acid -

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