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SHR Neuro Cancer Cardio Lipid Metab Microb

Niedrist, T; Kriegl, L; Zurl, CJ; Schmidt, F; Perkmann-Nagele, N; Mucher, P; Repl, M; Flieder, I; Radakovics, A; Sieghart, D; Radner, H; Aletaha, D; Binder, CJ; Gülly, C; Krause, R; Herrmann, M; Wagner, OF; Perkmann, T; Haslacher, H.
Preanalytical stability of SARS-CoV-2 anti-nucleocapsid antibodies.
Clin Chem Lab Med. 2023; 61(2):332-338 Doi: 10.1515/cclm-2022-0875
Web of Science PubMed FullText FullText_MUG


Leading authors Med Uni Graz
Niedrist Tobias
Co-authors Med Uni Graz
Gülly Christian
Herrmann Markus
Krause Robert
Kriegl Lisa
Zurl Christoph Johann

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OBJECTIVES: Anti-nucleocapsid (NC) antibodies are produced in response to SARS-CoV-2 infection. Therefore, they are well suited for the detection of a previous infection. Especially in the case of seroprevalence studies or during the evaluation of a novel in-vitro diagnostic test, samples have been stored at <-70 °C (short- and long-term) or 2-10 °C (short-term) before analysis. This study aimed to assess the impact of different storage conditions relevant to routine biobanking on anti-NC antibodies. METHODS: The preanalytical impact of short-term storage (84 [58-98] days) on <-70 °C and for 14 days at 2-10 °C was evaluated using samples from 111 donors of the MedUni Vienna Biobank. Long-term effects (443 [409-468] days) were assessed using 208 samples from Biobank Graz and 49 samples from Biobank Vienna. Anti-Nucleocapsid antibodies were measured employing electrochemiluminescence assays (Roche Anti-SARS-CoV-2). RESULTS: After short-term storage, the observed changes did not exceed the extent that could be explained by analytical variability. In contrast, results after long-term storage were approximately 20% higher and seemed to increase with storage duration. This effect was independent of the biobank from which the samples were obtained. Accordingly, the sensitivity increased from 92.6 to 95.3% (p=0.008). However, comparisons with data from Anti-Spike protein assays, where these deviations were not apparent, suggest that this deviation could also be explained by the analytical variability of the qualitative Anti-NC assay. CONCLUSIONS: Results from anti-NC antibodies are stable during short-term storage at <-70 °C and 2-10 °C. After long-term storage, a slight increase in sensitivity could not be ruled out.
Find related publications in this database (using NLM MeSH Indexing)
Humans - administration & dosage
SARS-CoV-2 - administration & dosage
Spike Glycoprotein, Coronavirus - administration & dosage
COVID-19 - diagnosis
Seroepidemiologic Studies - administration & dosage
Biological Specimen Banks - administration & dosage
Antibodies, Viral - administration & dosage
Sensitivity and Specificity - administration & dosage

Find related publications in this database (Keywords)
preanalytical phase
SARS-CoV-2 antibodies
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