Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Niedrist, T; Drexler, C; Torreiter, PP; Matejka, J; Strahlhofer-Augsten, M; Kral, S; Riegler, S; Gülly, C; Zurl, C; Kriegl, L; Krause, R; Berghold, A; Steinmetz, I; Schlenke, P; Herrmann, M.
Longitudinal Comparison of Automated SARS-CoV-2 Serology Assays in Assessing Virus Neutralization Capacity in COVID-19 Convalescent Sera.
Arch Pathol Lab Med. 2022; 146(5):538-546
Doi: 10.5858/arpa.2021-0604-SA
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Herrmann Markus
- Niedrist Tobias
- Co-authors Med Uni Graz
- Berghold Andrea
- Drexler-Helmberg Camilla
- Gülly Christian
- Kral Sabrina Barbara
- Krause Robert
- Kriegl Lisa
- Matejka Julia
- Riegler Skaiste
- Schlenke Peter
- Steinmetz Ivo
- Strahlhofer-Augsten Manuela Herta
- Torreiter Patrick Paul
- Zurl Christoph Johann
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- Abstract:
- CONTEXT.—: Serologic tests on automated immunology analyzers are increasingly used to monitor acquired immunity against SARS-CoV-2. The heterogeneity of assays raises concerns about their diagnostic performance and comparability. OBJECTIVE.—: To test sera from formerly infected individuals for SARS-CoV-2 antibodies by using 6 automated serology assays and a pseudoneutralization test (PNT). DESIGN.—: Six SARS-CoV-2 serology assays were used to assess 954 samples collected during a 12-month period from 315 COVID-19 convalescents. The tests determined either antibodies against the viral nucleocapsid (anti-NC) or spike protein (anti-S). Two assays did not distinguish between antibody classes, whereas the others selectively measured immunoglobulin G (IgG) antibodies. PNT was used to detect the presence of neutralizing antibodies. RESULTS.—: Comparison of qualitative results showed only slight to moderate concordance between the assays (Cohen κ < 0.57). Significant correlations (P < .001) were observed between the antibody titers from all quantitative assays. However, titer changes were not detected equally. A total anti-S assay measured an increase in 128 of 172 cases (74%) of a suitable subset, whereas all IgG anti-S tests reported decreases in at least 118 (69%). Regarding the PNT results, diagnostic sensitivities of 89% or greater were achieved with positive predictive values of at least 93%. In contrast, specificity changed substantially over time, varying from 20% to 100%. CONCLUSIONS.—: Comparability of serologic SARS-CoV-2 antibody tests is rather poor. Owing to different diagnostic specificities, the tested assays were not equally capable of capturing changes in antibody titers. However, with thoroughly validated cutoffs, IgG-selective anti-S assays are a reliable surrogate test for SARS-CoV-2 neutralizing antibodies in former COVID-19 patients.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies, Neutralizing - administration & dosage
- Antibodies, Viral - administration & dosage
- COVID-19 - diagnosis, therapy
- Humans - administration & dosage
- Immunization, Passive - administration & dosage
- Immunoglobulin G - administration & dosage
- SARS-CoV-2 - administration & dosage
- Sensitivity and Specificity - administration & dosage
- Spike Glycoprotein, Coronavirus - administration & dosage