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SHR Neuro Cancer Cardio Lipid Metab Microb

Bundschuh, C; Egger, M; Wiesinger, K; Gabriel, C; Clodi, M; Mueller, T; Dieplinger, B.
Evaluation of the EDI enzyme linked immunosorbent assays for the detection of SARS-CoV-2 IgM and IgG antibodies in human plasma.
Clin Chim Acta. 2020; 509(2):79-82 Doi: 10.1016/j.cca.2020.05.047 [OPEN ACCESS]
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Co-authors Med Uni Graz: GABRIEL Christian
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Abstract:: Besides SARS-CoV-2 RT-PCR testing, serological testing is emerging as additional option in COVID-19 diagnostics. Aim of this study was to evaluate novel immunoassays for detection of SARS-CoV-2 antibodies in human plasma. Using EDI^TM Novel Coronavirus COVID-19 Enzyme Linked Immunosorbent Assays (ELISAs), we measured SARS-CoV-2 IgM and IgG antibodies in 64 SARS-CoV-2 RT-PCR confirmed COVID-19 patients with serial blood samples (n = 104) collected at different time points from symptom onset. Blood samples from 200 healthy blood donors and 256 intensive care unit (ICU) patients collected before the COVID-19 outbreak were also used. The positivity rates in the COVID-19 patients were 5.9% for IgM and 2.9% for IgG ≤ 5 days after symptom onset; Between day 5 and day 10 the positivity rates were 37.1% for IgM and 37.1% for IgG and rose to 76.4% for IgM and 82.4% for IgG after > 10-15 days. After 15-22 days the "true" positivity rates were 94.4% for IgM and 100% for IgG. The "false" positivity rates were 0.5% for IgM and 1.0% for IgG in the healthy blood donors, 1.6% for IgM and 1.2% for IgG in ICU patients. This study shows high "true" vs. low "false" positivity rates for the EDI^TM SARS-CoV-2 IgM and IgG ELISAs. Copyright © 2020 Elsevier B.V. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing): Aged -; Aged, 80 and over -; Antibodies, Viral - blood; Betacoronavirus - isolation & purification; COVID-19 -; Coronavirus Infections - blood; Coronavirus Infections - diagnosis; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - standards; Female -; Humans -; Immunoglobulin G - blood; Immunoglobulin M - blood; Male -; Middle Aged -; Pandemics -; Pneumonia, Viral - blood; Pneumonia, Viral - diagnosis; SARS-CoV-2 -; Serologic Tests - methods; Serologic Tests - standards
Find related publications in this database (Keywords): COVID-19; SARS-CoV-2; ELISA; Antibody testing; Serological test