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SHR Neuro Cancer Cardio Lipid Metab Microb

Bundschuh, C; Egger, M; Wiesinger, K; Gabriel, C; Clodi, M; Mueller, T; Dieplinger, B.
Evaluation of the EDI enzyme linked immunosorbent assays for the detection of SARS-CoV-2 IgM and IgG antibodies in human plasma.
Clin Chim Acta. 2020; 509(2):79-82 Doi: 10.1016/j.cca.2020.05.047 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Co-authors Med Uni Graz
GABRIEL Christian

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Besides SARS-CoV-2 RT-PCR testing, serological testing is emerging as additional option in COVID-19 diagnostics. Aim of this study was to evaluate novel immunoassays for detection of SARS-CoV-2 antibodies in human plasma. Using EDITM Novel Coronavirus COVID-19 Enzyme Linked Immunosorbent Assays (ELISAs), we measured SARS-CoV-2 IgM and IgG antibodies in 64 SARS-CoV-2 RT-PCR confirmed COVID-19 patients with serial blood samples (n = 104) collected at different time points from symptom onset. Blood samples from 200 healthy blood donors and 256 intensive care unit (ICU) patients collected before the COVID-19 outbreak were also used. The positivity rates in the COVID-19 patients were 5.9% for IgM and 2.9% for IgG ≤ 5 days after symptom onset; Between day 5 and day 10 the positivity rates were 37.1% for IgM and 37.1% for IgG and rose to 76.4% for IgM and 82.4% for IgG after > 10-15 days. After 15-22 days the "true" positivity rates were 94.4% for IgM and 100% for IgG. The "false" positivity rates were 0.5% for IgM and 1.0% for IgG in the healthy blood donors, 1.6% for IgM and 1.2% for IgG in ICU patients. This study shows high "true" vs. low "false" positivity rates for the EDITM SARS-CoV-2 IgM and IgG ELISAs. Copyright © 2020 Elsevier B.V. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing)
Aged -
Aged, 80 and over -
Antibodies, Viral - blood
Betacoronavirus - isolation & purification
COVID-19 -
Coronavirus Infections - blood
Coronavirus Infections - diagnosis
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - standards
Female -
Humans -
Immunoglobulin G - blood
Immunoglobulin M - blood
Male -
Middle Aged -
Pandemics -
Pneumonia, Viral - blood
Pneumonia, Viral - diagnosis
SARS-CoV-2 -
Serologic Tests - methods
Serologic Tests - standards

Find related publications in this database (Keywords)
Antibody testing
Serological test
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