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Egger, M; Bundschuh, C; Wiesinger, K; Gabriel, C; Clodi, M; Mueller, T; Dieplinger, B.
Comparison of the Elecsys® Anti-SARS-CoV-2 immunoassay with the EDI™ enzyme linked immunosorbent assays for the detection of SARS-CoV-2 antibodies in human plasma.
Clin Chim Acta. 2020; 509(4):18-21 Doi: 10.1016/j.cca.2020.05.049 [OPEN ACCESS]
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Co-authors Med Uni Graz: GABRIEL Christian
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Abstract:: Here, we report on a head-to-head comparison of the fully-automated Elecsys® Anti-SARS-CoV-2 immunoassay with the EDI^TM enzyme linked immunosorbent assays (ELISA) for the detection of SARS-CoV-2 antibodies in human plasma. SARS-CoV-2 antibodies were measured with the Elecsys® assay and the EDI^TM ELISAs (IgM and IgG) in 64 SARS-CoV-2 RT-PCR confirmed COVID-19 patients with serial blood samples (n = 104) collected at different time points from symptom onset. Blood samples from 200 healthy blood donors and 256 intensive care unit (ICU) patients collected before the COVID-19 outbreak were also used. In COVID-19 patients, the percentage of positive results rose with time from symptom onset, peaking to positivity rates after 15-22 days of 100% for the Elecsys® assay, of 94% for the EDI^TM IgM-ELISA and of 100% for the EDI^TM IgG ELISA. In the 104 blood samples, the agreement between positive/negative classifications of the Elecsys® assay and the EDI^TM ELISAs (IgM or IgG) was 90%. The false positivity rates in the healthy blood donors and the ICU patients were < 1% for the Elecsys® assay and < 3% for the EDI^TM ELISAs. Our results indicate a high sensitivity and specificity for the Elecsys® assay and an acceptable agreement with the EDI^TM ELISAs. Copyright © 2020 Elsevier B.V. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing): Antibodies, Viral - blood; Betacoronavirus - isolation & purification; COVID-19 -; Clinical Laboratory Techniques - methods; Clinical Laboratory Techniques - standards; Coronavirus Infections - blood; Coronavirus Infections - diagnosis; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - standards; Female -; Humans -; Immunoassay - methods; Immunoassay - standards; Male -; Pandemics -; Pneumonia, Viral - blood; Pneumonia, Viral - diagnosis; SARS-CoV-2 -; Serologic Tests - methods; Serologic Tests - standards
Find related publications in this database (Keywords): COVID-19; SARS-CoV-2; ECLIA; Antibody testing; Serological test