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SHR Neuro Cancer Cardio Lipid

Bondarenko, AI; Montecucco, F; Panasiuk, O; Sagach, V; Sidoryak, N; Brandt, KJ; Mach, F.
GPR55 agonist lysophosphatidylinositol and lysophosphatidylcholine inhibit endothelial cell hyperpolarization via GPR-independent suppression of Na+-Ca2+ exchanger and endoplasmic reticulum Ca2+ refilling.
Vascul Pharmacol. 2017; 89(2):39-48
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Authors Med Uni Graz:
Bondarenko Oleksandr
Panasiuk Olga
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Abstract:
Lysophosphatidylinositol (LPI) and lysophosphatidylcholine (LPC) are lipid signaling molecules that induce endothelium-dependent vasodilation. In addition, LPC suppresses acetylcholine (Ach)-induced responses. We aimed to determine the influence of LPC and LPI on hyperpolarizing responses in vitro and in situ endothelial cells (EC) and identify the underlying mechanisms. Using patch-clamp method, we show that LPI and LPC inhibit EC hyperpolarization to histamine and suppress Na+/Ca2+ exchanged (NCX) currents in a concentration-dependent manner. The inhibition is non-mode-specific and unaffected by intracellular GDPβS infusion and tempol, a superoxide dismutase mimetic. In excised mouse aorta, LPI strongly inhibits the sustained and the peak endothelial hyperpolarization induced by Ach, but not by SKA-31, an opener of Ca2+-dependent K+ channels of intermediate and small conductance. The hyperpolarizing responses to consecutive histamine applications are strongly reduced by NCX inhibition. In a Ca2+-re-addition protocol, bepridil, a NCX inhibitor, and KB-R7943, a blocker of reversed NCX, inhibit the hyperpolarizing responses to Ca2+-re-addition following Ca2+ stores depletion. These finding indicate that LPC and LPI inhibit endothelial hyperpolarization to Ach and histamine independently of G-protein coupled receptors and superoxide anions. Reversed NCX is critical for ER Ca2+ refilling in EC. The inhibition of NCX by LPI and LPC underlies diminished endothelium-dependent responses and endothelial dysfunction accompanied by increased levels of these lipids in the blood. Copyright © 2017 Elsevier Inc. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing)
Acetylcholine - pharmacology
Animals -
Aorta, Thoracic - drug effects
Aorta, Thoracic - metabolism
Calcium Signaling - drug effects
Dose-Response Relationship, Drug -
Endoplasmic Reticulum - drug effects
Endoplasmic Reticulum - metabolism
Endothelial Cells - drug effects
Endothelial Cells - metabolism
Female -
Histamine - pharmacology
In Vitro Techniques -
Large-Conductance Calcium-Activated Potassium Channel alpha Subunits - agonists
Large-Conductance Calcium-Activated Potassium Channel alpha Subunits - metabolism
Lysophosphatidylcholines - pharmacology
Lysophospholipids - pharmacology
Male -
Membrane Potentials -
Mice, Inbred C57BL -
Receptors, Cannabinoid - drug effects
Receptors, Cannabinoid - metabolism
Sodium-Calcium Exchanger - antagonists & inhibitors
Sodium-Calcium Exchanger - metabolism
Vasodilation - drug effects
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Find related publications in this database (Keywords)
Lysophosphatidylinositol
Lysophosphatidylcholine
Endothelial cells
Hyperpolarization
Na+-Ca2+ exchanger
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