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Kormoczi, GF; Wolfel, UM; Rosenkranz, AR; Horl, WH; Oberbauer, R; Zlabinger, GJ.
Serum proteins modified by neutrophil-derived oxidants as mediators of neutrophil stimulation.
J IMMUNOL. 2001; 167(1): 451-460. [OPEN ACCESS]
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Authors Med Uni Graz:
Rosenkranz Alexander
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Abstract:
Reactive oxygen intermediates (ROI) released during inflammation may act as important mediators of neutrophil effector functions. The objective of this investigation was to evaluate the influence of ROI generation on neutrophil adhesion molecule regulation and degranulation. Induction of the neutrophil oxidative burst via Fcgamma receptor cross-linking was accompanied by up-regulation of neutrophil surface CD11b, CD35, and CD66b only in the presence of selected serum proteins, such as purified human C4, C5, or human serum albumin (HSA). Scavenging of ROI attenuated protein-dependent receptor regulations. Moreover, exogenous hydrogen peroxide was effective to increase neutrophil CD11b expression in a protein-dependent way. HSA exposed to neutrophil-derived ROI displayed signs of oxidative modification in terms of carbonyl formation. Such modified HSA transferred to resting neutrophils bound readily to the cell surface and effected receptor modulation as well as cellular spreading. In contrast, neither native HSA nor HSA protected against oxidation by the tocopherol analog Trolox exhibited agonistic properties. In conclusion, we demonstrate that neutrophil-derived ROI modify selected serum proteins, which, in turn, act as proinflammatory mediators of neutrophil stimulation.
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Blood Proteins - metabolism Blood Proteins - physiology
Complement C4 - physiology
Complement C5 - physiology
Complement C5a - physiology
Endopeptidases - blood Endopeptidases - physiology
Free Radical Scavengers - metabolism
Humans -
Hydrogen Peroxide - blood
Hydrolysis -
Immunoglobulin G - blood Immunoglobulin G - physiology
Interphase - physiology
Neutrophil Activation - physiology
Neutrophils - enzymology Neutrophils - metabolism Neutrophils - physiology
Oxidants - blood Oxidants - physiology
Protein Binding - physiology
Reactive Oxygen Species - metabolism Reactive Oxygen Species - physiology
Receptors, Cell Surface - blood
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Serum Albumin - metabolism Serum Albumin - physiology

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