Medizinische Universität Graz - Research portal
Selected Publication:
Ress, A.
MiR-96-5p level influences cellular growth and survival in colorectal cancer
Humanmedizin; [ Diplomarbeit ] Medical University of Graz; 2014. pp. 33
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- Authors Med Uni Graz:
- Advisor:
- Bauernhofer Thomas
- Pichler Martin
- Altmetrics:
- Abstract:
- Background: Expression of miR-96-5p is frequently altered in various types of cancer. However, the biological role of miR-96-5p expression in colorectal cancer (CRC) cells and its ability to predict the clinical course of patients has not been investigated yet. Methods: We explored miR-96-5p expression in 80 CRC patients and evaluated the impact on clinical outcome. In vitro miR-96-5p expression manipulation was performed in CRC cells and the effects on cellular growth, apoptosis and epithelial-mesenchymal transition (EMT)-related gene expression were explored. Results: Multivariate Cox regression analysis identified low levels of miR-96-5p as independent prognostic factors with respect to cancer-specific survival (hazard ratio=1.8, 95%CI=1.04-3.1, p<0.035). In vitro overexpression of miR-96-5p led to a reduced cellular growth rate (p<0.05), corroborated by a decreased cyclin D1 and increased p27- Cyclin-Dependent Kinase Inhibitor 1A (CDKN1A) expression (p<0.05). Forced expression of miR-96-5p in CRC cells entailed no effects on apoptosis or EMT-related genes but decreased the expression levels of the Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) oncogene (p<0.05). Despite regulating KRAS expression, there was no significant difference associated with miR-96-5p expression levels and response rates to Epidermal Growth Factor Receptor (EGFR) -targeting agents. Conclusion: Our data suggest that miR-96-5p influences cellular growth of CRC cells and low expression of miR-96-5p seems to be associated with poor clinical outcome in CRC patients.