Medizinische Universit├Ąt Graz - Research portal

Logo MUG Resarch Portal

Selected Publication:

GRAIER, WF; SCHMIDT, K; KUKOVETZ, WR.
ACTIVATION OF G-PROTEIN EVOKES CA2+ INFLUX IN ENDOTHELIAL-CELLS WITHOUT CORRELATION TO INOSITOL PHOSPHATES
J CARDIOVASC PHARMACOL. 1991; 17: S71-S78.
Web of Science PubMed FullText FullText_MUG Google Scholar

 

Authors Med Uni Graz:
Graier Wolfgang
Altmetrics:

Dimensions Citations:

Plum Analytics:
Abstract:
The production of big endothelin-1 (big ET-1) and its processing to endothelin (ET-1) were examined in cocultures of bovine pulmonary artery endothelial cells (BPECs) and human bronchiolar smooth muscle cells (HBSMCs). The BPECs produced immunologically reactive ET-1 (ir-ET-1) and big ET-1 (ir-big ET-1) that increased linearly in the cell culture media over a 72 h time course when confluent monolayers were incubated at 37 degrees C in serum-free media. HBSMCs maintained in serum-free media over a 72-h period did not produce any detectable ir-big ET-1 or ir-ET-1. After coculture of these two cell types for 24 h (the monolayers were physically separated), the culture medium contained no detectable ir-big ET-1 or ir-ET-1. When medium conditioned for 24 h with the HBSMCs was transferred to the BPEC monolayers, the accumulation rates of ET-1 and big ET-1 were attenuated 70 and 10%, respectively. When medium conditioned for 24 h with the BPECs was transferred to the HBSM cell monolayers, the amount of ir-ET-1 and ir-big ET-1 in the medium decreased greater than 90% over the next 24 h. Cellular binding and uptake could not account for this decrease as ascertained by quantitation of cellular levels of ET-1 and big ET-1. When conditioned medium from the HBSMCs was added to conditioned medium from the BPECs in a 1:1, 2:1, 3:1, 1:2, or 1:3 ratio (v/v) in the absence of cells, the amount of ET-1 or big ET-1 present in the EC medium did not decrease significantly over the following 24 h. These data imply that HBSMCs contain a factor that is responsible for decreasing the accumulation of ir-big ET-1 and ir-ET-1 in the cell culture media of BPECs. The data also suggest that the most likely mechanism for this effect is a factor that degrades BET-1 and ET-1 at different rates.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Bronchi - metabolism
Cattle -
Cells, Cultured -
Culture Media -
Endothelin-1 -
Endothelins - metabolism
Endothelium, Vascular - metabolism
Humans -
Muscle, Smooth - metabolism
Protein Precursors - metabolism
Pulmonary Artery - metabolism
Radioimmunoassay -

Find related publications in this database (Keywords)
ENDOTHELIAL CELL
G-PROTEIN
ENDOTHELIUM-DERIVED RELAXING FACTOR (EDRF)
CA2+ INFLUX
SODIUM FLUORIDE
IP3
IP4
© Med Uni GrazImprint