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SHR Neuro Cancer Cardio Lipid Metab Microb

Koppelstaetter, C; Jennings, P; Hochegger, K; Perco, P; Ischia, R; Karkoszka, H; Mayer, G.
Effect of tissue fixatives on telomere length determination by quantitative PCR.
Mech Ageing Dev. 2005; 126(12):1331-1333 Doi: 10.1016/j.mad.2005.08.003
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Co-authors Med Uni Graz
Eller Kathrin
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Abstract:
Telomere length is a well established marker of cellular senescence and thus biological age. Quantitative PCR allows the determination even from very low amounts of tissue by using telomere specific and single copy gene primers. Comparing a directly processed tissue sample to a 4% formaldehyde fixed one showed a significantly reduced efficiency of PCR reactions (mainly in single copy gene experiments) in a storage time-dependent manner resulting in an artificial increase in reported relative telomere length. This effect was not seen when the tissue was stored in RNA later solution. In summary, telomere length determination from formaldehyde fixed material by quantitative PCR is not a reliable method. Unfortunately therefore, many easily accessible tissue samples from pathology laboratories are unsuitable for this technique.
Find related publications in this database (using NLM MeSH Indexing)
Cell Aging -
DNA Primers - chemistry
Fixatives - pharmacology
Formaldehyde - pharmacology
Humans -
Paraffin - pharmacology
Polymerase Chain Reaction - methods
RNA - chemistry
Reverse Transcriptase Polymerase Chain Reaction -
Telomere - drug effects Telomere - ultrastructure
Time Factors -

Find related publications in this database (Keywords)
formaldehyde
RNA later
quantitative PCR
real time PCR
telomere length determination
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