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SHR Neuro Cancer Cardio Lipid

Bischof, H; Rehberg, M; Stryeck, S; Artinger, K; Eroglu, E; Waldeck-Weiermair, M; Gottschalk, B; Rost, R; Deak, AT; Niedrist, T; Vujic, N; Lindermuth, H; Prassl, R; Pelzmann, B; Groschner, K; Kratky, D; Eller, K; Rosenkranz, AR; Madl, T; Plesnila, N; Graier, WF; Malli, R.
Novel genetically encoded fluorescent probes enable real-time detection of potassium in vitro and in vivo.
Nat Commun. 2017; 8(1):1422-1422 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Authors Med Uni Graz:
Artinger Katharina
Bischof Helmut
Deak Andras Tamas
Eller Kathrin
Eroglu Emrah
Gottschalk Benjamin
Graier Wolfgang
Groschner Klaus
Kratky Dagmar
Lindermuth Hanna
Madl Tobias
Malli Roland
Niedrist Tobias
Pelzmann Brigitte
Prassl Ruth
Rosenkranz Alexander
Rost René
Stryeck Sarah
Vujic Nemanja
Waldeck-Weiermair Markus
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Number of Figures: 6
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Abstract:
Changes in intra- and extracellular potassium ion (K+) concentrations control many important cellular processes and related biological functions. However, our current understanding of the spatiotemporal patterns of physiological and pathological K+ changes is severely limited by the lack of practicable detection methods. We developed K+-sensitive genetically encoded, Förster resonance energy transfer-(FRET) based probes, called GEPIIs, which enable quantitative real-time imaging of K+ dynamics. GEPIIs as purified biosensors are suitable to directly and precisely quantify K+ levels in different body fluids and cell growth media. GEPIIs expressed in cells enable time-lapse and real-time recordings of global and local intracellular K+ signals. Hitherto unknown Ca2+-triggered, organelle-specific K+ changes were detected in pancreatic beta cells. Recombinant GEPIIs also enabled visualization of extracellular K+ fluctuations in vivo with 2-photon microscopy. Therefore, GEPIIs are relevant for diverse K+ assays and open new avenues for live-cell K+ imaging.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Biosensing Techniques - methods
Computer Systems -
Extracellular Fluid - metabolism
Fluorescence Resonance Energy Transfer -
Fluorescent Dyes -
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
HeLa Cells -
Humans -
In Vitro Techniques -
Intracellular Fluid - metabolism
Male -
Mice -
Mice, Inbred C57BL -
Microscopy, Fluorescence, Multiphoton -
Potassium - blood
Potassium - metabolism
Potassium - urine

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