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Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Valadez-Cosmes, P; Maitz, K; Kindler, O; Raftopoulou, S; Kienzl, M; Santiso, A; Mihalic, ZN; Brcic, L; Lindenmann, J; Fediuk, M; Pichler, M; Schicho, R; Houghton, AM; Heinemann, A; Kargl, J.
Identification of Novel Low-Density Neutrophil Markers Through Unbiased High-Dimensional Flow Cytometry Screening in Non-Small Cell Lung Cancer Patients.
Front Immunol. 2021; 12: 703846 Doi: 10.3389/fimmu.2021.703846 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Leading authors Med Uni Graz
Kargl Julia
Valadez Cosmes Paulina
Co-authors Med Uni Graz
Brcic Luka
Fediuk Melanie
Heinemann Akos
Kienzl Melanie
Kindler Oliver
Lindenmann Jörg
Maitz Kathrin
Mihalic Zala Nikita
Pichler Martin
Raftopoulou Sofia
Santiso Sanchez Ana Luisa
Schicho Rudolf

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Scite (citation analytics):

Neutrophils have been described as a phenotypically heterogeneous cell type that possess both pro- and anti-tumor properties. Recently, a subset of neutrophils isolated from the peripheral blood mononuclear cell (PBMC) fraction has been described in cancer patients. These low-density neutrophils (LDNs) show a heterogeneous maturation state and have been associated with pro-tumor properties in comparison to mature, high-density neutrophils (HDNs). However, additional studies are necessary to characterize this cell population. Here we show new surface markers that allow us to discriminate between LDNs and HDNs in non-small cell lung cancer (NSCLC) patients and assess their potential as diagnostic/prognostic tool. LDNs were highly enriched in NSCLC patients (median=20.4%, range 0.3-76.1%; n=26) but not in healthy individuals (median=0.3%, range 0.1-3.9%; n=14). Using a high-dimensional human cell surface marker screen, we identified 12 surface markers that were downregulated in LDNs when compared to HDNs, while 41 surface markers were upregulated in the LDN subset. Using flow cytometry, we confirmed overexpression of CD36, CD41, CD61 and CD226 in the LDN fraction. In summary, our data support the notion that LDNs are a unique neutrophil population and provide novel targets to clarify their role in tumor progression and their potential as diagnostic and therapeutic tool.

Find related publications in this database (Keywords)
low-density neutrophils
non-small cell lung cancer (NSCLC)
high-dimensional flow cytometry
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