Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Tiran, V; Stanzer, S; Heitzer, E; Meilinger, M; Rossmann, C; Lax, S; Tsybrovskyy, O; Dandachi, N; Balic, M.
Genetic profiling of putative breast cancer stem cells from malignant pleural effusions.
PLoS One. 2017; 12(4):e0175223-e0175223
Doi: 10.1371/journal.pone.0175223
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- Leading authors Med Uni Graz
- Balic Marija
- Dandachi Nadia
- Tiran Verena
- Co-authors Med Uni Graz
- Heitzer Ellen
- Meilinger Michael
- Roßmann Christopher Herbert
- Stanzer Stefanie
- Tsybrovskyy Oleksiy
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- Abstract:
- A common symptom during late stage breast cancer disease is pleural effusion, which is related to poor prognosis. Malignant cells can be detected in pleural effusions indicating metastatic spread from the primary tumor site. Pleural effusions have been shown to be a useful source for studying metastasis and for isolating cells with putative cancer stem cell (CSC) properties. For the present study, pleural effusion aspirates from 17 metastatic breast cancer patients were processed to propagate CSCs in vitro. Patient-derived aspirates were cultured under sphere forming conditions and isolated primary cultures were further sorted for cancer stem cell subpopulations ALDH1+ and CD44+CD24-/low. Additionally, sphere forming efficiency of CSC and non-CSC subpopulations was determined. In order to genetically characterize the different tumor subpopulations, DNA was isolated from pleural effusions before and after cell sorting, and compared with corresponding DNA copy number profiles from primary tumors or bone metastasis using low-coverage whole genome sequencing (SCNA-seq). In general, unsorted cells had a higher potential to form spheres when compared to CSC subpopulations. In most cases, cell sorting did not yield sufficient cells for copy number analysis. A total of five from nine analyzed unsorted pleura samples (55%) showed aberrant copy number profiles similar to the respective primary tumor. However, most sorted subpopulations showed a balanced profile indicating an insufficient amount of tumor cells and low sensitivity of the sequencing method. Finally, we were able to establish a long term cell culture from one pleural effusion sample, which was characterized in detail. In conclusion, we confirm that pleural effusions are a suitable source for enrichment of putative CSC. However, sequencing based molecular characterization is impeded due to insufficient sensitivity along with a high number of normal contaminating cells, which are masking genetic alterations of rare cancer (stem) cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Aged -
- Aged, 80 and over -
- Bone Neoplasms - diagnosis
- Bone Neoplasms - genetics
- Bone Neoplasms - metabolism
- Bone Neoplasms - secondary
- Breast Neoplasms - diagnosis
- Breast Neoplasms - genetics
- Breast Neoplasms - metabolism
- Breast Neoplasms - pathology
- Carcinoma, Ductal, Breast - diagnosis
- Carcinoma, Ductal, Breast - genetics
- Carcinoma, Ductal, Breast - metabolism
- Carcinoma, Ductal, Breast - secondary
- Cell Separation - instrumentation
- Cell Separation - methods
- DNA Copy Number Variations -
- DNA, Neoplasm - genetics
- Female -
- Flow Cytometry -
- Humans -
- Microsatellite Repeats -
- Middle Aged -
- Neoplastic Stem Cells - metabolism
- Neoplastic Stem Cells - pathology
- Pleural Effusion, Malignant - diagnosis
- Pleural Effusion, Malignant - genetics
- Pleural Effusion, Malignant - metabolism
- Pleural Effusion, Malignant - pathology
- Primary Cell Culture -
- Prospective Studies -
- Spheroids, Cellular - metabolism
- Spheroids, Cellular - pathology