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Wutte, N; Archelos, J; Crowe, BA; Zenz, W; Daghofer, E; Fazekas, F; Aberer, E.
Laboratory diagnosis of Lyme neuroborreliosis is influenced by the test used: comparison of two ELISAs, immunoblot and CXCL13 testing.
J Neurol Sci. 2014; 347(1-2):96-103
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Authors Med Uni Graz:
Aberer Elisabeth
Archelos-Garcia Juan-Jose
Daghofer Elisabeth
Fazekas Franz
Palfner-Wutte Nora Johanna
Zenz Werner
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Abstract:
To compare Borrelia-specific intrathecal antibodies by two different ELISAs, an immunoblot (IB) and CXCL13. Twenty-seven adults and 23 children with clinical symptoms compatible with NB were tested for Borrelia-specific intrathecal antibodies by flagellum ELISA-AI (flELISA), a recombinant ELISA-AI (rELISA) and by IB. Patients were classified according to the European Federation of Neurological Societies (EFNS) criteria as definite NB, possible NB, or non-NB. CSF CXCL13 levels were measured by ELISA. Among 50 patients, definite NB was diagnosed with the rELISA-AI in 29 (58%) patients, confirmed by IB in 19/29 patients, with flELISA-AI in 17 (34%) patients, confirmed by IB in 15/17 patients, and with IB in 20 (40%) patients. CXCL13 was positive in 22 (44%) patients. In 4 of 8 patients with negative AI, IB showed many detectable bands both in the CSF and serum. The diagnosis of NB strongly relies on the used test method. The rELISA-AI test appears to be the most sensitive while the flELISA-AI is the least sensitive. However when the ELISA-AIs were confirmed by IB, different patients were identified as NB, while only 26% were identified by all performed test methods. There is a demand for standardized test methods with well-defined sensitivity and specificity to establish validated diagnostic criteria for NB including the use of the IB assay and CXCL13 as an additional non-Borrelia specific determinant in early NB. Copyright © 2014 Elsevier B.V. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing)
Adolescent -
Adult -
Aged -
Borrelia burgdorferi - immunology
Chemokine CXCL13 - cerebrospinal fluid
Child -
Diagnosis, Differential -
Enzyme-Linked Immunosorbent Assay -
Female -
Humans -
Lyme Neuroborreliosis - cerebrospinal fluid
Lyme Neuroborreliosis - diagnosis
Lyme Neuroborreliosis - immunology
Male -
Middle Aged -
Predictive Value of Tests -
Sensitivity and Specificity -
Young Adult -

Find related publications in this database (Keywords)
CXCL13
Neuroborreliosis
Diagnosis
ELISA
Immunoblot
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