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SHR Neuro Cancer Cardio Lipid

Stadlbauer, V; Horvath, A; Komarova, I; Schmerboeck, B; Feldbacher, N; Wurm, S; Klymiuk, I; Durdevic, M; Rainer, F; Blesl, A; Stryeck, S; Madl, T; Stiegler, P; Leber, B.
A single alcohol binge impacts on neutrophil function without changes in gut barrier function and gut microbiome composition in healthy volunteers.
PLoS One. 2019; 14(2):e0211703-e0211703 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Authors Med Uni Graz:
Blesl Andreas
Durdevic Marija
Horvath Angela
Klymiuk Ingeborg
Komarova Irina
Leber Bettina
Madl Tobias
Rainer Florian
Schmerböck Bianca
Stadlbauer-Köllner Vanessa
Stiegler Philipp
Stryeck Sarah
Wurm Sonja
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Abstract:
Alcohol binge drinking is a dangerous drinking habit, associated with neurological problems and inflammation. The impact of a single alcohol binge on innate immunity, gut barrier and gut microbiome was studied. In this cohort study 15 healthy volunteers received 2 ml vodka 40% v/v ethanol/kg body weight. Neutrophil function was studied by flow cytometry; markers of gut permeability and inflammation (lactulose/mannitol/sucrose test, zonulin, calprotectin, diamino-oxidase) were studied with NMR spectroscopy and enzyme-linked immunosorbent assay in urine, stool and serum respectively. Bacterial products in serum were quantified using different reporter cell lines. Gut microbiome composition was studied by 16S rDNA sequencing and bioinformatics analysis. After a single alcohol binge, neutrophils were transiently primed and the response to E.coli stimulation with reactive oxygen species (ROS) production was transiently increased, on the other hand the percentage of neutrophils that did not perform phagocytosis increased. No changes in gut permeability, inflammatory biomarker, bacterial translocation and microbiome composition could be detected up to 4 hours after a single alcohol binge or on the next day. A single alcohol binge in young, healthy volunteers transiently impacts on neutrophil function. Although the exact biological consequence of this finding is not clear yet, we believe that this strengthens the importance to avoid any alcohol binge drinking, even in young, otherwise healthy persons.

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