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Selected Publication:

Buzina, W; Lang-Loidolt, D; Braun, H; Freudenschuss, K; Stammberger, H.
Development of molecular methods for identification of Schizophyllum commune from clinical samples.
J CLIN MICROBIOL. 2001; 39(7): 2391-2396. Doi: 10.1128/JCM.39.7.2391-2396.2001 [OPEN ACCESS]
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Leading authors Med Uni Graz
Buzina Walter
Co-authors Med Uni Graz
Braun Hannes
Lang-Loidolt Doris
Stammberger Heinz
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Abstract:
In the last 50 years, to our knowledge, only 16 cases of diseases caused by Schizophyllum commune in humans have been reported. Within only 6 months, we found four isolates of this basidiomycetous fungus, obtained from patients suffering from chronic sinusitis. The cultures of the isolated fungi showed neither clamp connections nor fruiting bodies (basidiocarps), which are distinctive features for S. commune, but fast-growing cottony white mycelium only. This was harvested, and DNA was extracted. The internal transcribed spacer region of the ribosomal DNA (rDNA) was amplified with fungus-specific primers, and the PCR products were sequenced. Two strains of S. commune, collected from branches of a European hornbeam (Carpinus betulus) and a tree of heaven (Ailanthus altissima), respectively; four specimens from the herbarium of the Institute of Botany, Karl-Franzens-University Graz; and two strains from internationally known culture collections (CBS 340.81 [ATCC 44201] and CBS 405.96) were investigated in the same way. The sequence data of all strains were compared and showed homology of over 99% in this 660-bp-long fragment of rDNA. With these results, a map of restriction enzyme cutting sites and a primer set specific for S. commune were created for reliable identification of this human pathogenic fungus.
Find related publications in this database (using NLM MeSH Indexing)
Base Sequence -
DNA, Fungal - analysis
DNA, Ribosomal Spacer - genetics
Female - genetics
Humans - genetics
Middle Aged - genetics
Molecular Sequence Data - genetics
Mycological Typing Techniques - genetics
Mycoses - microbiology
Polymerase Chain Reaction - microbiology
Schizophyllum - classification
Sequence Analysis, DNA - classification

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