Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Leber, B; Stadlbauer, V; Stiegler, P; Stanzer, S; Mayrhauser, U; Koestenbauer, S; Leopold, B; Sereinigg, M; Puntschart, A; Stojakovic, T; Tscheliessnigg, KH; Oettl, K.
Effect of oxidative stress and endotoxin on human serum albumin in brain-dead organ donors.
Transl Res. 2012; 159(6):487-496
Doi: 10.1016/j.trsl.2011.12.005
Web of Science
PubMed
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FullText_MUG
- Leading authors Med Uni Graz
- Leber Bettina
- Stadlbauer-Köllner Vanessa
- Stiegler Philipp
- Co-authors Med Uni Graz
- Köstenbauer Sonja
- Leopold-Posch Barbara
- Mayrhauser Ursula
- Öttl Karl
- Puntschart Andreas Jürgen
- Sereinigg Michael
- Stanzer Stefanie
- Stojakovic Tatjana
- Tscheliessnigg Karlheinz
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- Abstract:
- Albumin, among other molecules, binds and detoxifies endotoxin in healthy people. Oxidative stress leads to protein oxidation and thus to the impaired binding properties of albumin. This property, in combination with increased gut permeability, leads to the appearance of endotoxin in the systemic circulation and to impaired organ function. We hypothesize that these processes occur in the serum of brain-dead organ donors. Endotoxin was determined with an adapted Limulus amoebocyte lysate assay. The albumin fractions and binding capacity were determined by high-performance liquid chromatography (HPLC). FlowCytomix (eBioscience, San Diego, Calif) was used to determine the cytokine levels. Carbonylated proteins (CPs) and myeloperoxidase (MPO) were measured by an enzyme-linked immunosorbent assay (ELISA). Eighty-four brain-dead organ donors were enrolled and categorized by the duration of intensive care unit (ICU) stay. The albumin-binding capacity for dansylsarcosine was reduced in brain-dead patients compared with controls. Endotoxin positivity in 16.7% of donors was associated with decreased binding capacity in donors and worse survival of recipients. The CP and MPO levels of organ donors were significantly higher than in healthy controls. The durations of ICU stay increased albumin oxidation. In addition, interleukin-6 (IL-6), IL-8, IL-10, and IL-1β levels were increased in patients, whereas the interferon-γ (IFN-γ) levels were within the normal range. We conclude that oxidative stress and systemic endotoxemia are present in brain-dead organ donors, which might affect recipient survival. High endotoxin levels might be caused by increased gut permeability and decreased binding capacity of albumin influenced not just by higher albumin oxidation.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Aged -
- Brain Death - blood
- Cytokines - blood
- Endotoxins - blood
- Female -
- Humans -
- Intensive Care -
- Interleukins - blood
- Kaplan-Meier Estimate -
- Male -
- Middle Aged -
- Oxidative Stress -
- Peroxidase - blood
- Protein Binding -
- Protein Carbonylation -
- Retrospective Studies -
- Serum Albumin - metabolism
- Time Factors -
- Tissue Donors -
- Tissue and Organ Harvesting -
- Translational Medical Research -
- Transplants -
- Young Adult -