Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Mayrhauser, U; Stiegler, P; Stadlbauer, V; Koestenbauer, S; Leber, B; Konrad, K; Iberer, F; Tscheliessnigg, K.
Cell to cell interactions influence sensitivity of liver cell lines during hyperthermia.
Anticancer Res. 2011; 31(11):3713-3717
Web of Science
PubMed
- Leading authors Med Uni Graz
- Mayrhauser Ursula
- Stiegler Philipp
- Co-authors Med Uni Graz
- Iberer Florian
- Konrad Katja Alena
- Köstenbauer Sonja
- Leber Bettina
- Stadlbauer-Köllner Vanessa
- Tscheliessnigg Karlheinz
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- Abstract:
- Background: Thermal cancer therapy is used for hepatocellular carcinoma treatment. In this study we investigated the effect of hyperthermia on liver cells and compared data of our different cell culture fibrosis models (transwell vs. co-culture model). Materials and Methods: The cell lines HepG2 and LX-1 were seeded in different numbers in transwells to simulate different grades of fibrosis and then heated from 55 degrees C to 85 degrees C for different time spans. Thereafter, metabolic activity was measured. Results: Heating at 65 degrees C showed that the greater the number of LX-1 cells treated together with HepG2 cells the lower the metabolic activity of HepG2 cells was. Compared to our previous co-culture study, there were significantly different results in cell survival from 55 degrees C to 75 degrees C. Conclusion: The co-culture fibrosis model is more physiological than the transwell model because it allows a higher seeding density and a higher degree of cell to cell interactions. Therefore, it is more efficient for investigating the effect of hyperthermia on liver cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology Carcinoma, Hepatocellular - therapy
- Cell Communication -
- Cell Proliferation -
- Cells, Cultured -
- Coculture Techniques -
- Fibrosis - metabolism Fibrosis - pathology
- Hepatic Stellate Cells - metabolism Hepatic Stellate Cells - pathology
- Humans -
- Hyperthermia, Induced -
- Liver Neoplasms - metabolism Liver Neoplasms - pathology Liver Neoplasms - therapy
- Find related publications in this database (Keywords)
- Hyperthermia
- thermal cancer therapy
- hepatic stellate cells
- fibrosis
- HCC
- transwell
- co-culture