Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Mayrhauser, U; Stiegler, P; Stadlbauer, V; Koestenbauer, S; Leber, B; Konrad, K; Iberer, F; Portugaller, RH; Tscheliessnigg, K.
Effect of hyperthermia on liver cell lines: important findings for thermal therapy in hepatocellular carcinoma.
Anticancer Res. 2011; 31(5):1583-1588
Web of Science
PubMed
- Leading authors Med Uni Graz
- Mayrhauser Ursula
- Stiegler Philipp
- Co-authors Med Uni Graz
- Iberer Florian
- Konrad Katja Alena
- Köstenbauer Sonja
- Leber Bettina
- Portugaller Rupert
- Stadlbauer-Köllner Vanessa
- Tscheliessnigg Karlheinz
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- Abstract:
- Background: Hepatocellular carcinoma is the fifth most common malignant tumour, with a high mortality rate. This study aimed to investigate the effect of hyperthermia on HepG2 and LX-1 cell lines to gain more information on thermal treatment of liver tumours. Materials and Methods: The cell lines HepG2, LX-1 and their co-cultures were heated from 55 degrees C to 85 degrees C for different time spans. After heat exposure, metabolic activity was measured immediately, and after 24 h and 48 h using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) test to assess how many cells had survived heating. Results: Our results show highly significant differences between the temperature tolerance of HepG2 and LX-1 cells. Alone, HepG2 cells are most sensitive to heat-induced cell death, their sensitivity decreased with rising percentages of LX-1 cells in the co-culture. Conclusion: Our results suggest that the outcome of thermal cancer therapy is dependent on the temperature and the grade of fibrosis in the treated livers.
- Find related publications in this database (using NLM MeSH Indexing)
- Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology Carcinoma, Hepatocellular - therapy
- Coculture Techniques -
- Fever -
- Hot Temperature - therapeutic use
- Humans -
- Liver - metabolism
- Liver Neoplasms - metabolism Liver Neoplasms - pathology Liver Neoplasms - therapy
- Tetrazolium Salts - pharmacology
- Thiazoles - pharmacology
- Tumor Cells, Cultured -
- Find related publications in this database (Keywords)
- Hyperthermia
- thermal cancer therapy
- hepatic stellate cells
- fibrosis
- HCC
- HepG2