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SHR Neuro Krebs Kardio Lipid

Ball, T; Linhart, B; Sonneck, K; Blatt, K; Herrmann, H; Valent, P; Stoecklinger, A; Lupinek, C; Thalhamer, J; Fedorov, AA; Almo, SC; Valenta, R.
Reducing allergenicity by altering allergen fold: a mosaic protein of Phl p 1 for allergy vaccination.
Allergy. 2009; 64(4):569-580 [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Ball Tanja
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Abstract:
BACKGROUND: The major timothy grass pollen allergen, Phl p 1, resembles the allergenic epitopes of natural group I grass pollen allergens and is recognized by more than 95% of grass-pollen-allergic patients. Our objective was the construction, purification and immunologic characterization of a genetically modified derivative of the major timothy grass pollen allergen, Phl p 1 for immunotherapy of grass pollen allergy. METHODS: A mosaic protein was generated by PCR-based re-assembly and expression of four cDNAs coding for Phl p 1 fragments and compared to the Phl p 1 wild-type by circular dichroism analysis, immunoglobulin E (IgE)-binding capacity, basophil activation assays and enzyme-linked immunosorbent assay competition assays. Immune responses to the derivative were studied in BALB/c mice. RESULTS: Grass-pollen-allergic patients exhibited greater than an 85% reduction in IgE reactivity to the mosaic as compared with the Phl p 1 allergen and basophil activation experiments confirmed the reduced allergenic activity of the mosaic. It also induced less Phl p 1-specific IgE antibodies than Phl p 1 upon immunization of mice. However, immunization of mice and rabbits with the mosaic induced IgG antibodies that inhibited patients' IgE-binding to the wild-type allergen and Phl p 1-induced degranulation of basophils. CONCLUSION: We have developed a strategy based on rational molecular reassembly to convert one of the clinically most relevant allergens into a hypoallergenic derivative for allergy vaccination.
Find related publications in this database (using NLM MeSH Indexing)
Adult -
Aged -
Allergens - biosynthesis Allergens - chemistry Allergens - immunology
Amino Acid Sequence -
Animals -
Basophils - immunology Basophils - secretion
Desensitization, Immunologic - methods
Epitopes, T-Lymphocyte - chemistry Epitopes, T-Lymphocyte - immunology
Female -
Histamine - biosynthesis Histamine - immunology
Humans -
Immunoglobulin E - blood Immunoglobulin E - immunology
Immunoglobulin G - blood Immunoglobulin G - immunology
Lymphocyte Activation - immunology
Male -
Mice -
Mice, Inbred BALB C -
Middle Aged -
Molecular Sequence Data -
Plant Proteins - biosynthesis Plant Proteins - chemistry Plant Proteins - immunology
Polymerase Chain Reaction -
Protein Structure, Quaternary -
Rabbits -
Rats -
Recombinant Proteins - biosynthesis Recombinant Proteins - chemical synthesis Recombinant Proteins - immunology
Rhinitis, Allergic, Seasonal - immunology Rhinitis, Allergic, Seasonal - prevention and control
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization -
T-Lymphocytes - immunology

Find related publications in this database (Keywords)
allergens
immunotherapy
pollens
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