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Majumder, S; Tamilarasan, KP; Kolluru, GK; Muley, A; Nair, CM; Omanakuttan, A; Murty, KV; Chatterjee, S.
Activated pericyte attenuates endothelial functions: nitric oxide-cGMP rescues activated pericyte-associated endothelial dysfunctions.
Biochem Cell Biol. 2007; 85(6):709-720
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Autor/innen der Med Uni Graz:
Kuppusamy Palaniappan Tamilarasan
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Abstract:
Hepatic stellate cells are liver-specific pericytes and exist in close proximity with endothelial cells. The activation of liver pericytes is intrinsic to liver pathogenesis, and leads to endothelial dysfunction, including the low bioavailability of nitric oxide (NO). However, the role of nitric oxide in pericyte-endothelium cross-talk has not yet been elucidated. This work examines the cellular mechanism of action of NO in pericyte-mediated endothelial dysfunction. We used in vitro coculture and conditioned medium systems to study the effects of activated liver pericytes on endothelial function, and an egg yolk vascular bed model was used to study the effects of activated pericytes on angiogenesis. This study also demonstrates that activated pericytes attenuate the migration, proliferation, permeability, and NO production of endothelial cells. Our results demonstrate that activated pericytes restrict angiogenesis in egg yolk vascular bed models, and NO supplementation recovers 70% of the inhibition. Our results also demonstrate that supplementation with NO, sildenafil citrate (phosphodiesterase inhibitor), and 8-bromo-cGMP (cGMP analog) partially recovers activated-pericyte-mediated endothelium dysfunction. We conclude that NO-cGMP alleviates activated-pericyte-associated endothelial dysfunction, including angiogenesis, in a cGMP-dependent manner.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Cell Line -
Cell Membrane Permeability - drug effects
Cell Movement - drug effects
Cell Proliferation - drug effects
Cell Surface Extensions - drug effects
Chickens - drug effects
Culture Media, Conditioned - pharmacology
Cyclic GMP - metabolism
Dose-Response Relationship, Drug - metabolism
Endothelial Cells - cytology
Enzyme Inhibitors - pharmacology
Guanylate Cyclase - antagonists and inhibitors
Hepatocytes - cytology
Humans - cytology
Hydrolysis - drug effects
Neovascularization, Physiologic - drug effects
Nitric Oxide - biosynthesis
Pericytes - cytology
Solubility - drug effects
Wound Healing - drug effects

Find related publications in this database (Keywords)
hepatic stellate cells
endothelial cells
nitric oxide
cGMP
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