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Gewählte Publikation:

Tatzber, F; Griebenow, S; Wonisch, W; Winkler, R.
Dual method for the determination of peroxidase activity and total peroxides-iodide leads to a significant increase of peroxidase activity in human sera.
Anal Biochem. 2003; 316(2):147-153
Web of Science PubMed

 

Autor/innen der Med Uni Graz:
Tatzber Franz
Wonisch Willibald
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Abstract:
Peroxidases are very important enzymes, e.g., as preventive antioxidants by removing noxious peroxides from the blood. For this reason we evaluated a colorimetric method which detects the activity of endogenous peroxidases by their reaction with hydrogen peroxide, using tetramethylbenzidine as the chromogenic substrate. This assay design can be easily reversed by change of the variable compound to measure also total peroxides in plasma or serum. An increased total antioxidant status was reported previously by the addition of iodide to human serum. In this study iodide activated the endogenous peroxidases significantly in comparison to control sera and isomolar NaCl as well as horseradish peroxidase. Corresponding to the increased peroxidase activity a concomitant decrease of total peroxides occurred in the same samples. This exchangeable assay design is a beneficial opportunity to screen total peroxide levels as well as peroxidase activity in human sera without time-consuming preparations. The method proved to be simple and is favorable due to its specificity, reproducibility, and low costs. Moreover, we were able to find an explanation for the increased total antioxidant status in the presence of iodide, which is presumably an indirect protective effect via an enhanced activity of enzymatic antioxidants, thereby reducing endogenous peroxides.
Find related publications in this database (using NLM MeSH Indexing)
Adult -
Aged -
Antioxidants - analysis
Colorimetry - methods
Enzyme Activation - drug effects
Humans -
Iodide Peroxidase - analysis
Iodide Peroxidase - blood
Iodides - chemistry
Middle Aged -
Peroxidase - analysis
Peroxidase - blood
Peroxides - analysis
Peroxides - blood
Reproducibility of Results -
Serum - chemistry
Sodium Chloride - pharmacology
Sodium Iodide - pharmacology

Find related publications in this database (Keywords)
iodine
oxidative stress
lipid peroxidation
peroxidases
peroxide
total antioxidant status
salt
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