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Gewählte Publikation:

Stöcher, M; Leb, V; Bozic, M; Kessler, HH; Halwachs-Baumann, G; Landt, O; Stekel, H; Berg, J.
Parallel detection of five human herpes virus DNAs by a set of real-time polymerase chain reactions in a single run.
J Clin Virol. 2003; 26(1):85-93
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Autor/innen der Med Uni Graz:
Berg Jörg
Bozic Michael
Halwachs-Baumann Gabriele
Kessler Harald
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Abstract:
BACKGROUND: Human herpes viruses cause a spectrum of diseases that are usually self-limiting but can be reactive during immuno-suppression and may then lead to severe or even life-threatening diseases. The LightCycler technology allows rapid polymerase chain reaction (PCR) including product analysis within a closed system. This approach has been demonstrated to be suitable for routine diagnostic virus detection. Several LightCycler PCR assays have been established to the detection of human herpes viruses. The assays vary in their detection formats and PCR cycling protocols. So, they cannot be performed within a single LightCycler run. OBJECTIVES: Development of four LightCycler PCR assays for parallel detection of DNA derived from human cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), and varicella-zoster virus (VZV) in a single run. STUDY DESIGN: Primers and hybridization probes were tailored to suit one LightCycler PCR program. LightCycler PCRs were established, detection limits were determined, and clinical samples were evaluated. RESULTS: With quantified herpes virus type specific DNA spiked into cerebrospinal fluid, serum or EDTA plasma the detection limits were found either at 500 or 250 viral DNA copies per ml depending on the virus DNA specific PCR and on the specimen type used. The applicability of the new LightCycler assays for routine molecular testing was evaluated by testing 96 clinical samples. CONCLUSION: The developed set of LightCycler PCRs permits parallel detection of CMV, EBV, HSV-1, HSV-2, and VZV in a single LightCycler run. The new molecular assays can easily be used to the rapid, simple, and convenient detection of herpes virus DNA in cerebrospinal fluid, serum and EDTA plasma in the routine diagnostic laboratory.
Find related publications in this database (using NLM MeSH Indexing)
Anticoagulants -
Blood - virology
Blood Specimen Collection -
Cerebrospinal Fluid - virology
Computer Systems -
Cytomegalovirus - genetics
DNA, Viral - analysis DNA, Viral - blood DNA, Viral - cerebrospinal fluid
Edetic Acid -
Herpesviridae - genetics
Herpesvirus 1, Human - genetics
Herpesvirus 3, Human - genetics
Herpesvirus 4, Human - genetics
Humans -
Polymerase Chain Reaction - instrumentation Polymerase Chain Reaction - methods
Sensitivity and Specificity -
Species Specificity -

Find related publications in this database (Keywords)
cytomegalovirus (CMV)
Epstein-Barr virus (EBV)
herpes simplex virus (HSV)
varicella-zoster virus (VZV)
real-time polymerase chain reaction (PCR)
LightCycler
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