Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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Gewählte Publikation:

Stubbs, VE; Schratl, P; Hartnell, A; Williams, TJ; Peskar, BA; Heinemann, A; Sabroe, I.
Indomethacin causes prostaglandin D(2)-like and eotaxin-like selective responses in eosinophils and basophils.
J BIOL CHEM 2002 277: 26012-26020. [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Heinemann Akos
Luschnig Petra
Peskar Bernhard
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Abstract:
We investigated the actions of a panel of nonsteroidal anti-inflammatory drugs on eosinophils, basophils, neutrophils, and monocytes. Indomethacin alone was a potent and selective inducer of eosinophil and basophil shape change. In eosinophils, indomethacin induced chemotaxis, CD11b up-regulation, respiratory burst, and L-selectin shedding but did not cause up-regulation of CD63 expression. Pretreatment of eosinophils with indomethacin also enhanced subsequent eosinophil shape change induced by eotaxin, although treatment with higher concentrations of indomethacin resulted in a decrease in the expression of the major eosinophil chemokine receptor, CCR3. Indomethacin activities and cell selectivity closely resembled those of prostaglandin D(2) (PGD(2)). Eosinophil shape change in response to eotaxin was inhibited by pertussis toxin, but indomethacin- and PGD(2)-induced shape change responses were not. Treatment of eosinophils with specific inhibitors of phospholipase C (U-73122), phosphatidylinositol 3-kinase (LY-294002), and p38 mitogen-activated protein kinase (SB-202190) revealed roles for these pathways in indomethacin signaling. Indomethacin and its analogues may therefore provide a structural basis from which selective PGD(2) receptor small molecule antagonists may be designed and which may have utility in the treatment of allergic inflammatory disease.
Find related publications in this database (using NLM MeSH Indexing)
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Antigens, CD - metabolism
Basophils - drug effects
Chemokines, CC - metabolism
Chemotaxis, Leukocyte - drug effects
Chromones - drug effects
Enzyme Inhibitors - pharmacology
Eosinophils - drug effects
Estrenes - pharmacology
Humans - pharmacology
Imidazoles - pharmacology
Indomethacin - pharmacology
Macrophage-1 Antigen - metabolism
Mitogen-Activated Protein Kinases - antagonists and inhibitors
Morpholines - antagonists and inhibitors
Pertussis Toxin - antagonists and inhibitors
Phospholipase C - antagonists and inhibitors
Platelet Membrane Glycoproteins - metabolism
Prostaglandin D2 - metabolism
Pyridines - pharmacology
Pyrrolidinones - pharmacology
Respiratory Burst - pharmacology
Up-Regulation - pharmacology
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p38 Mitogen-Activated Protein Kinases - pharmacology

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