Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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Gewählte Publikation:

Kadyrov, M; Kaufmann, P; Huppertz, B.
Expression of a cytokeratin 18 neo-epitope is a specific marker for trophoblast apoptosis in human placenta.
PLACENTA 2001 22: 44-48.
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Autor/innen der Med Uni Graz:
Huppertz Berthold
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Abstract:
In epithelial cells the caspase-mediated cleavage of cytokeratin 18 during apoptosis leads to the formation of a specific neo-epitope, recognized by the antibody M30. To test whether this antibody can be used as a specific marker for apoptotic trophoblast, we have stained serial sections of villi and junctional zone of first and third trimester human placenta with antibodies against cytokeratins 7 and 18, and against active caspase 3, with M30 and with the TUNEL reaction. Comparison of M30 immunoreactivities with TUNEL positivity and immunoreactivities for cytokeratins 7 and 18 clearly demonstrates that M30 specifically labels late apoptotic trophoblast cells. This finding is supported by the fact that in trophoblast, M30 immunoreactivities largely overlap with those for active caspase 3. As compared to the TUNEL test, the M30 immune reaction appears to be a highly reproducible marker for apoptotic trophoblast. This antibody stains a larger number of cells within the apoptosis cascade as compared to the TUNEL reaction, since cytokeratin 18 cleavage starts earlier than cleavage of DNA and since endonuclease activation can be bypassed in some trophoblast cells. The data suggest that M30 is superior to the TUNEL reaction as a marker for the detection of trophoblast apoptosis since it is easier to handle, more specific for apoptosis and less prone to artifacts.
Find related publications in this database (using NLM MeSH Indexing)
Adult -
Antibodies, Monoclonal -
Apoptosis -
Biological Markers - analysis
Chorionic Villi - metabolism
Epitope Mapping - metabolism
Female - metabolism
Fluorescent Antibody Technique, Indirect - metabolism
Humans - metabolism
In Situ Nick-End Labeling - metabolism
Keratin - immunology
Pregnancy - immunology
Pregnancy Trimester, First - immunology
Research Support, Non-U.S. Gov't - immunology
Trophoblasts - metabolism

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