Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Direkt zur Navigaton springen.
Direkt zum Inhalt springen.

Navigation/Suche

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid Stoffw Microb

Gottschalk, B; Koshenov, Z; Bachkoenig, OA; Rost, R; Malli, R; Graier, WF.
MFN2 mediates ER-mitochondrial coupling during ER stress through specialized stable contact sites.
Front Cell Dev Biol. 2022; 10: 918691 Doi: 10.3389/fcell.2022.918691 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

Führende Autor*innen der Med Uni Graz: Gottschalk Benjamin; Graier Wolfgang
Co-Autor*innen der Med Uni Graz: Bachkönig Olaf Arne Georg; Koshenov Zhanat; Malli Roland; Rost René
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: Endoplasmic reticulum (ER) functions critically depend on a suitable ATP supply to fuel ER chaperons and protein trafficking. A disruption of the ability of the ER to traffic and fold proteins leads to ER stress and the unfolded protein response (UPR). Using structured illumination super-resolution microscopy, we revealed increased stability and lifetime of mitochondrial associated ER membranes (MAM) during ER stress. The consequent increase of basal mitochondrial Ca²⁺ leads to increased TCA cycle activity and enhanced mitochondrial membrane potential, OXPHOS, and ATP generation during ER stress. Subsequently, OXPHOS derived ATP trafficking towards the ER was increased. We found that the increased lifetime and stability of MAMs during ER stress depended on the mitochondrial fusion protein Mitofusin2 (MFN2). Knockdown of MFN2 blunted mitochondrial Ca²⁺ effect during ER stress, switched mitochondrial F₁F_O-ATPase activity into reverse mode, and strongly reduced the ATP supply for the ER during ER stress. These findings suggest a critical role of MFN2-dependent MAM stability and lifetime during ER stress to compensate UPR by strengthening ER ATP supply by the mitochondria.
Find related publications in this database (Keywords): mitochondria; ER stress; mitochondrial Ca2+; mitofusin 2; mitochondria-associated membranes (MAM)