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SHR Neuro Krebs Kardio Lipid

Kasatkina, LA; Gumenyuk, VP; Sturm, EM; Heinemann, A; Bernas, T; Trikash, IO.
Modulation of neurosecretion and approaches for its multistep analysis.
BBA-GEN SUBJECTS. 2018; 1862(12): 2701-2713.
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Autor/innen der Med Uni Graz:
Heinemann Akos
Sturm Eva
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Abstract:
Neurosecretion is the multistep process occurring in separate spatial and temporal cellular boundaries which complicates its comprehensive analysis. Most of the research are focused on one distinct stage of synaptic vesicle recycling. Here, we describe approaches for complex analysis of synaptic vesicle (SV) endocytosis and separate steps of exocytosis at the level of presynaptic bouton and highly purified SVs. Proposed fluorescence-based strategies and analysis of neurotransmitter transport provided the advantages in studies of exocytosis steps. We evaluated SV docking/tethering, their Ca2+-dependent fusion and release of neurotransmitters gamma-aminobutyric acid (GABA) and glutamate in two animal models. Approaches enabled us to study: 1) endocytosis/Ca2+-dependent release of fluorescent carbon nanodots (CNDs) during stimulation of nerve terminals; 2) the action of levetiracetam, modulator of SV glycoprotein SV2, on fusion competence of SVs and stimulated release of GABA and glutamate; 3) impairments of several steps of neurosecretion under vitamin D3 deficiency. Our algorithm enabled us to verify the method validity for multidimensional analysis of SV turnover. By increasing SV docking and the size of readily releasable pool (RRP), levetiracetam is able to selectively enhance the stimulated GABA secretion in hippocampal neurons. Findings suggest that SV2 regulates RRP through impact on the number of docked/primed SVs. Methodology can be widely applied to study the stimulated neurosecretion in presynapse, regulation of SV docking, their Ca2+-dependent fusion with target membranes, quantitative analysis of expression of neuron-specific proteins, as well as for testing the efficiency of pre-selected designed neuroactive substances. Copyright © 2018 Elsevier B.V. All rights reserved.

Find related publications in this database (Keywords)
Synaptic vesicles recycling
Confocal imaging
Carbon nanodots
Vitamin D-3 deficiency
SV2 protein
GABA release
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