Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
von Zur Mühlen, C; Koeck, T; Schiffer, E; Sackmann, C; Zürbig, P; Hilgendorf, I; Reinöhl, J; Rivera, J; Zirlik, A; Hehrlein, C; Mischak, H; Bode, C; Peter, K.
Urine proteome analysis as a discovery tool in patients with deep vein thrombosis and pulmonary embolism.
PROTEOM CLIN APPL. 2016; 10(5): 574-584.
Doi: 10.1002/prca.201500105
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Zirlik Andreas
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- Abstract:
- Early and accurate detection of deep vein thrombosis (DVT) is an important clinical need. Based on the hypothesis that urinary peptides may hold information on DVT in conjunction with pulmonary embolism (PE), the study was aimed at identifying such peptide biomarkers using capillary electrophoresis coupled mass spectrometry. Patients with symptoms of unprovoked/idiopathic DVT and/or PE were examined by doppler-sonography or angio-computed tomography. Urinary proteome analysis allowed for identification of respective peptide biomarkers. To confirm their biological relevance, we induced PE in mice and assessed human ex vivo thrombi. We identified 62 urinary peptides as DVT-specific biomarkers, i.e. fragments of collagen type I and a fragment of fibrinogen β-chain. The presence of fibrinogen α/β in the acute thrombus, and collagen type I and osteopontin in the older, organized thrombus was demonstrated. The classifier DVT62 established through support vector machine (SVM) modeling based on the 62 identified peptides was validated in an independent cohort of 47 subjects (six cases and 41 controls) with a sensitivity of 100% and specificity of 83%. Urine proteome analysis enabled the detection of DVT-specific peptides, which were validated in human and mouse tissue. Furthermore, it allowed for the establishment of an urinary-proteome based classifier that is relatively specific for DVT. The data provide the basis for assessment of these biomarkers in a prospective clinical study. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Animals -
- Biomarkers - urine
- Case-Control Studies -
- Collagen Type I - genetics
- Collagen Type I - urine
- Electrophoresis, Capillary -
- Female -
- Fibrinogen - genetics
- Fibrinogen - urine
- Gene Expression -
- Humans -
- Male -
- Mass Spectrometry -
- Mice -
- Mice, Inbred C57BL -
- Middle Aged -
- Osteopontin - genetics
- Osteopontin - urine
- Proteome - metabolism
- Pulmonary Embolism - complications
- Pulmonary Embolism - diagnosis
- Pulmonary Embolism - genetics
- Pulmonary Embolism - urine
- Sensitivity and Specificity -
- Support Vector Machine -
- Urinalysis - instrumentation
- Urinalysis - methods
- Venous Thrombosis - complications
- Venous Thrombosis - diagnosis
- Venous Thrombosis - genetics
- Venous Thrombosis - urine
- Find related publications in this database (Keywords)
- Biomarker
- Discovery Tool
- Pulmonary Embolism
- Thrombosis