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SHR Neuro Krebs Kardio Lipid

Pfanzagl, B; Mechtcheriakova, D; Meshcheryakova, A; Aberle, SW; Pfragner, R; Jensen-Jarolim, E.
Activation of the ileal neuroendocrine tumor cell line P-STS by acetylcholine is amplified by histamine: role of H3R and H4R.
Sci Rep. 2017; 7(1):1313-1313 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Autor/innen der Med Uni Graz:
Pfragner Roswitha

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Number of Figures: 7
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Neuroendocrine tumors may present with pseudoallergic reactions like diarrhea and idiopathic anaphylaxis. Here we present the P-STS human ileal neuroendocrine cell line as a model cell line for these tumors. Neuroendocrine markers and changes in cytoplasmic calcium concentration ([Ca2+]i) in response to several possible activators of 5-hydroxytryptamine (5-HT) release were analyzed. P-STS cells still expressed chromogranin A and synaptophysin after 2 years of culture. Tryptophan hydroxylase 1 mRNA and a low amount of 5-HT were also detected. Acetylcholine (ACh) caused a rise in [Ca2+]i. Somatostatin inhibited, whereas histamine (HA) but not the HA receptor ligand betahistine enhanced activation by ACh. The [Ca2+]i response to ACh/HA was inhibited by the HA receptor H3 (H3R) agonist methimepip and by the antidepressant imipramine. Further [Ca2+]i response studies indicated the presence of H4Rs and of a functional calcium sensing receptor. High or low affinity IgE receptor protein or mRNA were not detected. Taken together, neuroendocrine markers and response to intestinal neurotransmitters approve the P-STS cell line as a valuable model for enterochromaffin cells. Enhancement of their ACh-induced pro-secretory response by HA, with a role for H3R and H4R, suggests an amplifying role of neuroendocrine cells in allergen-induced diarrhea or anaphylaxis.
Find related publications in this database (using NLM MeSH Indexing)
Acetylcholine - pharmacology
Betahistine - pharmacology
Calcium - metabolism
Cell Line, Tumor -
Chromogranin A - pharmacology
Gene Expression Regulation, Neoplastic - drug effects
Histamine - genetics
Histamine - metabolism
Humans -
Ileal Neoplasms - drug therapy
Ileal Neoplasms - genetics
Ileal Neoplasms - pathology
Neuroendocrine Tumors - drug therapy
Neuroendocrine Tumors - genetics
Neuroendocrine Tumors - pathology
Receptors, Histamine H3 - genetics
Receptors, Histamine H3 - metabolism
Receptors, Histamine H4 - genetics
Receptors, Histamine H4 - metabolism
Serotonin - genetics
Somatostatin - pharmacology
Synaptophysin - pharmacology
Tryptophan Hydroxylase - genetics

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