Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Birkl, C; Soellradl, M; Toeglhofer, AM; Krassnig, S; Leoni, M; Pirpamer, L; Vorauer, T; Krenn, H; Haybaeck, J; Fazekas, F; Ropele, S; Langkammer, C.
Effects of concentration and vendor specific composition of formalin on postmortem MRI of the human brain.
Magn Reson Med. 2018; 79(2):1111-1115
Doi: 10.1002/mrm.26699
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- Führende Autor*innen der Med Uni Graz
- Birkl Christoph
- Co-Autor*innen der Med Uni Graz
- Birkl-Töglhofer Anna Maria
- Fazekas Franz
- Haybäck Johannes
- Kraßnig Stefanie
- Langkammer Christian
- Leoni Marlene
- Pirpamer Lukas
- Ropele Stefan
- Söllradl Martin
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- Abstract:
- Formalin fixation prevents tissue autolysis by crosslinking proteins and changes tissue microstructure and MRI signal characteristics. Previous studies showed high variations in MR relaxation time constants of formalin fixed brain tissue, which has been attributed to the use of different formalin concentrations. Our investigations confirmed the influence of formalin concentration on relaxation times and unexpectedly revealed an influence of vendor specific formalin composition, which has not been investigated so far. We systematically analyzed relaxation times of human brain tissue fixed with 4% and 10% formalin compared with unfixed condition at 3 Tesla MRI. Furthermore, we assessed relaxation times of nine formalin solutions from different vendors and performed comparisons of their magnetic susceptibility by SQUID (superconducting quantum interference device) magnetometry. Tissue relaxation times decreased approximately twice as fast using 10% than in 4% formalin fixation. The vendor specific composition of the formalin solutions and concentration dependent paramagnetic effects showed a substantial contribution to differences in relaxation times of formalin. Our study demonstrates that differences of the formalin composition have substantial effects on MRI signal characteristics after fixation, which can explain the divergence of reported relaxation times beyond the effect of differences in formalin concentration. Magn Reson Med 79:1111-1115, 2018. © 2017 International Society for Magnetic Resonance in Medicine. © 2017 International Society for Magnetic Resonance in Medicine.
- Find related publications in this database (Keywords)
- formalin fixation
- formalin concentration
- postmortem MRI
- formalin fixed brain tissue
- relaxation properties of formalin
- magnetic properties of formalin