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SHR Neuro Krebs Kardio Lipid

Üçal, M; Kraitsy, K; Weidinger, A; Paier-Pourani, J; Patz, S; Fink, B; Molcanyi, M; Schäfer, U.
Comprehensive Profiling of Modulation of Nitric Oxide Levels and Mitochondrial Activity in the Injured Brain: An Experimental Study Based on the Fluid Percussion Injury Model in Rats.
J Neurotrauma. 2017; 34(2):475-486
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Autor/innen der Med Uni Graz:
Kraitsy Klaus
Molcanyi Marek
Patz Silke
Schäfer Ute
Ücal Muammer
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Abstract:
Nitric oxide (NO) has frequently been associated with secondary damage after brain injury. However, average NO levels in different brain regions before and after traumatic brain injury (TBI) and its role in post-TBI mitochondrial dysfunction remain unclear. In this comprehensive profiling study, we demonstrate for the first time that basal NO levels vary significantly in the healthy cortex (0.44 ± 0.04 μM), hippocampus (0.26 ± 0.03 μM), and cerebellum (1.24 ± 0.08 μM). Within 4 h of severe lateral fluid percussion injury, NO levels almost doubled in these regions, thereby preserving regional differences in NO levels. TBI-induced NO generation was associated with inducible NO synthase (iNOS) increase in ipsilateral but not in contralateral regions. The transient NO increase resulted in a persistent tyrosine nitration adjacent to the injury site. Nitrosative stress-associated cell loss via apoptosis and receptor-interacting serine/threonine-protein kinase 3 (RIPK3)-mediated necrosis were also observed in the ipsilateral cortex, despite high levels of NO in the contralateral cortex. NO-mediated impairment of mitochondrial state 3 respiration dependent on complex I substrates was transient and confined to the ipsilateral cortex. Our results demonstrate that NO dynamics and associated effects differ in various regions of the injured brain. A potential association between the observed mitochondrial electron flow through complex I, but not complex II, and the modulation of TBI induced NO levels in different brain regions has to be prospectively analyzed in more detail.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Brain Injuries - genetics
Brain Injuries - metabolism
Brain Injuries - pathology
Cerebral Cortex - injuries
Cerebral Cortex - metabolism
Cerebral Cortex - pathology
Disease Models, Animal -
Gene Expression Profiling - methods
Glutamic Acid - genetics
Glutamic Acid - metabolism
Male -
Mitochondria - genetics
Mitochondria - metabolism
Mitochondria - pathology
Nitric Oxide - metabolism
Percussion - methods
Random Allocation -
Rats -
Rats, Sprague-Dawley -

Find related publications in this database (Keywords)
brain injury
fluid percussion
glutamate
mitochondria
NO
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