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SHR Neuro Krebs Kardio Lipid

Enko, D; Kriegshäuser, G; Stolba, R; Worf, E; Halwachs-Baumann, G.
Method evaluation study of a new generation of vitamin D assays.
Biochem Med (Zagreb). 2015; 25(2):203-212 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Autor/innen der Med Uni Graz:
Enko Dietmar
Halwachs-Baumann Gabriele
Kriegshäuser Gernot

Dimensions Citations:

Plum Analytics:
Number of Figures: 3
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Recently several diagnostic manufacturers have launched new 25-hydroxy-vitamin D (25[OH]D) assays, which are aligned to the National Institute of Standards and Technology (NIST) Standard Reference Materials (SRM) (NIST, Gaithersburg, Maryland). The aim of this study was to compare the performance of one liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, one enzyme linked immunosorbent assay (ELISA), and one recalibrated and previous version of a chemiluminescence immunoassay (CLIA). Serum-aliquots of 198 patient samples from routine 25(OH)D analysis were measured by the ClinMass® LC-MS/MS Complete Kit (RECIPE Chemicals+Instruments GmbH, Munich, Germany), the ORGENTEC 25(OH)D3/D2 ELISA (ORGENTEC Diagnostika GmbH, Mainz, Germany), the recalibrated Immunodiagnostic Systems (IDS)-iSYS 25(OH)DS and the previous used IDS-iSYS 25(OH)D CLIA (Immunodiagnostic Systems Ltd, Boldon, United Kingdom). Bland-Altman and Deming regression analyses were calculated for methods comparison of all tested 25(OH)D assays. The LC-MS/MS method was defined as the reference method. Within-run and between-run precision measurements were performed for all methods with three different concentration levels. Compared to the LC-MS/MS method, the new IDS-iSYS 25(OH)DS and ORGENTEC 25(OH)D3/D2 assay demonstrated mean relative biases of 16.3% and 17.8%. The IDS-iSYS 25(OH)D assay showed the lowest mean bias of 1.5%. Deming regression analyses of the recalibrated IDS-iSYS 25(OH)DS and the ORGENTEC 25(OH)D3/D2 assay showed proportional differences, when compared to the reference method. All assays showed a within-run and between-run imprecision of ≤20% at each of the evaluated concentration levels. The evaluated standardized immunoassays and LC-MS/MS are useful methods for measuring 25(OH)D serum-levels in clinical laboratories.
Find related publications in this database (using NLM MeSH Indexing)
Chromatography, Liquid - methods
Chromatography, Liquid - standards
Female -
Humans -
Male -
Mass Spectrometry - methods
Mass Spectrometry - standards
Reference Standards -
Vitamin D - analogs & derivatives
Vitamin D - blood

Find related publications in this database (Keywords)
vitamin D
liquid chromatography-tandem mass spectrometry
reference standards
quality improvement
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